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辽河高凝油微生物采油菌剂研究及应用评价

发布时间:2018-01-19 00:06

  本文关键词: 高凝油 微生物采油技术 嗜热脂肪地芽孢杆菌 嗜热脱氮地芽孢杆菌 铜绿假单胞菌 出处:《岩性油气藏》2017年05期  论文类型:期刊论文


【摘要】:针对高凝油含蜡高、凝固点高、流动性差及开采难度大的问题,选用铜绿假单胞菌配合嗜热脂肪地芽孢杆菌和嗜热脱氮地芽孢杆菌,采用四组分分析法和饱和烃气相色谱法等方法开展了微生物提高高凝油采收率菌剂研究和应用评价。结果表明:菌种对原油四组分存在选择性降解,降解率为23.0%~42.3%,同时菌种可以将高凝油中长碳链饱和烃降解为短碳链烃类,w(nC_(21))/w(nC_(22))值和w(nC_(21)+nC_(22))/w(nC_(28)+nC_(29))值增大0.33~0.57;铜绿假单胞菌发酵液表面张力从72.21 mN/m降低至26.81 mN/m;嗜热脂肪地芽孢杆菌与嗜热脱氮地芽孢杆菌2种芽孢杆菌乳化高凝油的E24值分别为70.6%和82.3%;基于嗜热脂肪地芽孢杆菌、嗜热脱氮地芽孢杆菌和铜绿假单胞菌3种细菌性能设计的兼容本源微生物的复合微生物采油菌剂可使高凝油黏度降低63.86%,凝固点降低6℃。物理模拟驱油实验表明:该微生物复合菌剂可在中渗(200 mD)及低渗(50 mD)条件下使高凝油采收率提高6.46%~8.48%。6口油井的微生物吞吐采油试验证明该微生物复合菌剂性能稳定,可使高凝油采收率大幅提高,具有良好的工业应用前景。
[Abstract]:In order to solve the problems of high wax content, high freezing point, poor fluidity and difficulty in mining, Pseudomonas aeruginosa was selected in combination with Bacillus thermophilus and Bacillus thermophilus. Four component analysis method and saturated hydrocarbon gas chromatography method were used to study and evaluate microorganism to improve oil recovery. The results showed that the bacteria could selectively degrade the four components of crude oil. The degradation rate is 23.0 ~ 42.3%, and the strain can degrade the long chain saturated hydrocarbon into the short carbon chain hydrocarbon in the high coagulant oil. / / / the / / /. The value increased by 0.33 ~ 0.57; The surface tension of Pseudomonas aeruginosa fermentation broth decreased from 72.21 mN/m to 26.81 mn / m; The E24 values of Bacillus thermophilus and Bacillus thermophilus were 70.6% and 82.3 respectively. Based on Bacillus thermophilus, Bacillus thermophilus and Pseudomonas aeruginosa, a compound microbial oil recovery agent compatible with native microorganisms could reduce the viscosity of high coagulant oil by 63.86%. The solidification point was reduced by 6 鈩,

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