基于高通量测序技术研究玉北油田上方微生物多样性

发布时间：2018-01-28 00:03

  本文关键词： 油气微生物勘探 高通量测序 群落结构 微生物多样性　出处：《应用与环境生物学报》2017年02期 　论文类型：期刊论文

【摘要】：精确识别活性微生物标识物是目前微生物油气勘探的技术核心,为深入认识各典型油气藏上方油气微生物标识物,采用高通量测序技术研究玉北地区油气富集带上方的油气微生物物种丰度、分布、群落结构等特征.结果显示油气点上方样品较之背景非油气点具有更多的特有物种,其中油气点样品中含199种,背景非油气点样品中含77种.从中共鉴定出10种主要油气微生物,发现其中的3类优势物种(假单胞菌属、芽孢杆菌属和土壤杆菌属)是利用烃类兼性生长的微生物;同时通过解析统计学差异值较高的生物标记物时,发现两种统计学差异值大于5的微生物,分别为背景非油气点上的弧菌科Vibrionaceae维氏气单胞菌和油气点上的方肠杆菌科Erythrobacteraceae赤杆菌.本研究可为油气微生物勘探提供直接实验依据.
[Abstract]:Accurate identification of active microbial markers is the core of microbial oil and gas exploration technology, in order to understand the typical oil and gas reservoirs above the oil and gas microbial markers. High throughput sequencing technique was used to study the abundance and distribution of microbial species above the oil-gas enrichment zone in Yubei area. The results show that the samples above the oil and gas point have more endemic species than the background non-oil and gas spots, among which 199 species are found in the oil and gas point samples. Background there are 77 species in non-oil and gas samples. Ten main oil and gas microbes were identified and 3 dominant species (Pseudomonas) were found. Bacillus and Agrobacterium) are microbes that use hydrocarbon facultative growth; At the same time, by analyzing the biomarkers with high statistical difference, we found two kinds of microbes with statistical difference value greater than 5. They are Vibrionaceae Vickers on the background and Erythrobacteraceae gibberellae on the oil and gas sites respectively. The results of this study are as follows:. Oil and gas microbial exploration provides direct experimental basis.
【作者单位】： 浙江大学地球科学系;中国石油化工股份有限公司石油勘探开发研究院无锡石油地质研究所;
【基金】：中国石化股份公司科技部项目(P14042) 国家自然科学基金项目(41202241,41372131,41172112)资助~~
【分类号】：P618.13;P632.4
【正文快照】： 油气微生物勘探技术(Microbial Prospecting for Oil andGas,MPOG)是研究近地表土壤层中微生物异常与地下深部油气藏关系的地表勘探法的一个分支[1].油气微生物勘探从属于地质微生物学.它是以地质学和生物学,特别是地球化学和微生物学为基础而发展起来的交叉边缘学科.经过60多，

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