固定化脂肪酶催化桐油制备生物柴油的研究

发布时间：2018-04-09 16:12

  本文选题：磁性FeO纳米粒　切入点：固定化脂肪酶　出处：《中国油脂》2017年01期

【摘要】：对磁性Fe3O4纳米粒固定化脂肪酶催化桐油制备生物柴油进行了研究,分步探讨了硼酸盐缓冲液用量、固定化酶用量、醇油摩尔比、反应温度、固定化酶清洗与否对转酯反应的影响,以及固定化酶的操作稳定性。结果表明:将正己烷与桐油体积比定为2∶1,然后加入与桐油等摩尔的甲醇、桐油体积6%的硼酸盐缓冲液及7.5 mg/m L(以桐油体积计)固定化酶,反应5 h和12 h各加入与桐油等摩尔的甲醇(总的醇油摩尔比3∶1),并每次添加甲醇前用丙酮清洗固定化酶,45℃、200 r/min反应26 h后,甲酯转化率可达91.2%。该固定化脂肪酶连续催化10批次反应后,甲酯转化率仍然可达84.1%,具有一定的工业应用价值。
[Abstract]:The preparation of biodiesel from tung oil catalyzed by immobilized lipase of magnetic Fe3O4 nanoparticles was studied. The amount of borate buffer, the amount of immobilized enzyme, the molar ratio of alcohol to oil, the reaction temperature were studied step by step.The effect of immobilized enzyme cleaning on transesterification and the operation stability of immobilized enzyme.The results showed that the volume ratio of n-hexane to tung oil was 2: 1, then methanol was added to tung oil, borate buffer was 6% by volume of tung oil and enzyme was immobilized at 7.5 mg/m / L (by tung oil volume).After 5 h and 12 h respectively adding methanol (the total molar ratio of alcohol to oil 3: 1) with tung oil, and cleaning the immobilized enzyme with acetone for 26 h before adding methanol, the conversion rate of methyl ester can reach 91.2%.After 10 batches of continuous catalytic reaction with the immobilized lipase, the conversion rate of methyl ester can still reach 84.1, which has certain industrial application value.
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本文编号：1727171