富含果胶的工业富产物生产微生物油脂初步研究

发布时间：2018-05-09 21:13

本文选题：微生物油脂 + 果胶　；参考：《大连工业大学》2015年硕士论文

【摘要】：由于化石燃料的短缺和生态环境的恶化,寻找可替代的生物柴油原料在全球范围内引起广泛关注。近年来,人们逐步认识到微生物油脂是生物柴油产业发展的重要替代原料。由于培养产油微生物的原料对微生物油脂的经济性有重要影响,研究高效利用廉价生物质资源制备油脂十分必要。在果蔬加工和制糖工业过程中,产生大量富含果胶的残渣,属于廉价废弃的生物质资源。果胶是一种组成和结构复杂的多糖,其水解产物主要为半乳糖醛酸,另外还含阿拉伯糖和葡萄糖等。由于典型工业微生物如酿酒酵母Saccharomyces cerevisiae和大肠杆菌Escherichia coli等不能高效利用半乳糖醛酸和阿拉伯糖等单糖,富含果胶的生物质资源尚未能作为发酵工业的原料得到应用。因此,本论文拟研究产油酵母转化利用半乳糖醛酸的性能,为富含果胶生物质资源的高值化利用提供科学依据。为探索产油酵母利用富含果胶生物质资源的能力,本文利用10株产油酵母以半乳糖醛酸为碳源进行评估,发现Trichosporon cutaneum AS 2.571,Trichosporon fermentans CICC 1368和Cryptococcus curvatus ATCC 20509三株菌可有效地将半乳糖醛酸转化为油脂,油脂得率均达0.12 g/g。以模拟甜菜渣水解液,即半乳糖醛酸、阿拉伯糖和葡萄糖的混合物作为碳源培养产油酵母,发现T.cutaneum AS 2.571和T.fermentans CICC 1368的油脂得率达0.15 g/g以上。上述三株产油酵母利用甜菜渣水解液进行油脂发酵实验,C.curvatus ATCC 20509油脂含量可达到43.6%,油脂得率为0.14 g/g还原糖。发酵得到的油脂的脂肪酸成分均与植物油相似。结果表明,富含果胶的生物质可作为微生物油脂生产的原料。
[Abstract]:Because of the shortage of fossil fuels and the deterioration of ecological environment, the search for alternative biodiesel feedstock has attracted worldwide attention. In recent years, it has been gradually recognized that microbial oil is an important substitute for the development of biodiesel industry. Because the feedstock of oil-producing microorganism has an important influence on the economy of microbial oil, it is necessary to study the efficient use of cheap biomass resources to produce oil. In the process of fruit and vegetable processing and sugar industry, a large amount of pectin rich residue is produced, which is a cheap waste biomass resource. Pectin is a kind of polysaccharide with complex composition and structure. Its hydrolytic product is galacturonic acid, in addition to arabinose and glucose. Because typical industrial microorganisms such as Saccharomyces cerevisiae Saccharomyces cerevisiae and Escherichia coli Escherichia coli can not efficiently utilize monosaccharides such as galacturonic acid and arabinose, the biomass resources rich in pectin have not been used as raw materials for fermentation industry. Therefore, this paper intends to study the conversion and utilization of galacturonic acid by oil-producing yeast, and provide scientific basis for the high value utilization of rich pectin biomass resources. In order to explore the ability of oil-producing yeast to utilize rich pectin biomass resources, 10 oil-producing yeasts were evaluated with galacturonic acid as carbon source. It was found that three strains of Trichosporon cutaneum AS 2.571Trichosporon fermentans CICC 1368 and Cryptococcus curvatus ATCC 20509 could effectively convert galacturonic acid into oil, and the oil yield was 0.12 g / g. Using simulated beet residue hydrolysate, that is, the mixture of galacturonic acid, arabinose and glucose as carbon source, the oil yield of T.cutaneum as 2.571 and T.fermentans CICC 1368 was found to be more than 0.15 g / g. The oil content of C. curvatus ATCC 20509 was 43.6% and the oil yield was 0.14 g / g reducing sugar. The fatty acid composition of the fermented oil was similar to that of the vegetable oil. The results showed that the biomass rich in pectin could be used as raw material for microbial oil production.
【学位授予单位】：大连工业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TE667

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