菌株P.aeruginosa BC1的筛

发布时间：2018-05-22 20:57

  本文选题：铜绿假单胞菌 + 鼠李糖脂　； 参考：《油田化学》2016年04期

【摘要】：为改善克拉玛依油田的微生物强化原油开采,从克拉玛依油田废水中筛选出一株性能优良的生物表面活性剂生产菌株BC1。根据菌株BC1的理化指标和16S r DNA对其进行了属种信息鉴定,通过薄层层析(TLC)、傅里叶红外色谱(FT-IR)及高效液相色谱/质谱(HPLC-MS)对其产生物表面活性剂进行了分析,通过单因素摇瓶实验考察了发酵条件对菌株BC1发酵合成生物表面活性剂的影响,研究了生物表面活性剂的性能。研究结果表明,菌株BC1为铜绿假单胞菌属(Pseudomonas aeruginosa),其产生物表面活性剂主要成分为单鼠李糖脂Rh C10C10;在温度37℃、初始p H为8、初始葡萄糖浓度5 g/L的条件下持续发酵96 h后,用酸沉降法测得鼠李糖脂产量为0.788 g/L;菌株BC1产生物表面活性剂能将发酵液表面张力从72降至28.6 m N/m,对车用机油的乳化指数E24高达93%。人工油砂剥油实验中,发酵液、发酵离心上清液和鼠李糖脂粗品溶液对人工油砂的剥油率均达到74%以上。菌株P.aeruginosa BC1产生物表面活性剂在微生物强化采油等方面具有良好的应用潜能。
[Abstract]:In order to improve microbial enhanced crude oil production in Karamay Oilfield, a strain BC1 with excellent performance was screened from the wastewater of Karamay Oilfield. According to the physicochemical index of strain BC1 and the information of 16s r DNA, the surfactants were analyzed by TLC, FTIR and HPLC-MS. The effects of fermentation conditions on biosurfactant synthesis by strain BC1 were investigated by single factor flask shaking experiment. The properties of biosurfactants were studied. The results showed that the strain BC1 was Pseudomonas aeruginosaformis, and the main component of its surfactant was rhamnolipid Rh C10C10, after fermentation for 96 h at 37 鈩，

本文编号：1923577