华北宝力格油田水处理系统微生物群落结构及多样性分析
发布时间：2018-08-13 15:12
【摘要】：在华北宝力格油田水处理系统的三相分离器与反应池之间加入激活剂,采用16S r DNA高通量测序技术,研究三相分离器、反应池、过滤器和喂水泵4个水处理节点水样中微生物(细菌和古菌)群落结构及多样性,并分析不同水处理节点水样中驱油功能菌含量。研究结果表明,各水处理节点水样的细菌和古菌群落结构存在差异。三相分离器、反应池、过滤器和喂水泵处水样中细菌群落中的优势菌分别为弯曲菌科、红长命菌属、红长命菌属和固氮弧菌属、地衣芽孢杆菌属;三相分离器和喂水泵处水样中古菌群落中的优势菌均为甲烷食甲基菌属。喂水泵处细菌和古菌多样性较前一个水处理节点减小,说明微生物群落结构由复杂向简单发展。喂水泵处驱油功能菌含量最大,这些驱油功能菌注入地下后可发挥驱油作用。
[Abstract]:The activator was added between the three-phase separator and the reaction cell in the water treatment system of North China Baolige Oilfield, and the 16s r DNA high-throughput sequencing technique was used to study the three-phase separator and reaction cell. The community structure and diversity of microbes (bacteria and archaea) in the water samples of four water treatment nodes of filter and feed water pump were analyzed, and the content of oil displacement function bacteria in the water samples of different water treatment nodes were analyzed. The results showed that the community structure of bacteria and Archaea were different between the water samples of different water treatment nodes. The dominant bacteria in the water samples of three-phase separator, reaction tank, filter and feed water pump were Campylobacteriaceae, Rhodovibrio, Vibrio nitrogen-fixing, Bacillus licheniformis. The dominant bacteria in the water samples of three-phase separator and feed pump were methane-feeding methyl bacteria. The diversity of bacteria and archaea in the feed pump is smaller than that in the former water treatment node, which indicates that the microbial community structure has changed from complex to simple. The oil displacement function bacteria in the water pump are the largest, which can play a role in oil displacement after they are injected underground.
【作者单位】： 华东理工大学化学与分子工程学院;中国石油华北油田分公司采油工程研究院;中国石油大学(华东)理学院;
【基金】：中国石油重大科技专项“华北油田上产稳产800万吨关键技术研究与应用”(2014E-3507)
【分类号】：TE357.9
本文编号：2181355
[Abstract]:The activator was added between the three-phase separator and the reaction cell in the water treatment system of North China Baolige Oilfield, and the 16s r DNA high-throughput sequencing technique was used to study the three-phase separator and reaction cell. The community structure and diversity of microbes (bacteria and archaea) in the water samples of four water treatment nodes of filter and feed water pump were analyzed, and the content of oil displacement function bacteria in the water samples of different water treatment nodes were analyzed. The results showed that the community structure of bacteria and Archaea were different between the water samples of different water treatment nodes. The dominant bacteria in the water samples of three-phase separator, reaction tank, filter and feed water pump were Campylobacteriaceae, Rhodovibrio, Vibrio nitrogen-fixing, Bacillus licheniformis. The dominant bacteria in the water samples of three-phase separator and feed pump were methane-feeding methyl bacteria. The diversity of bacteria and archaea in the feed pump is smaller than that in the former water treatment node, which indicates that the microbial community structure has changed from complex to simple. The oil displacement function bacteria in the water pump are the largest, which can play a role in oil displacement after they are injected underground.
【作者单位】： 华东理工大学化学与分子工程学院;中国石油华北油田分公司采油工程研究院;中国石油大学(华东)理学院;
【基金】：中国石油重大科技专项“华北油田上产稳产800万吨关键技术研究与应用”(2014E-3507)
【分类号】：TE357.9
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1 孙会梅;石油污染物质输入对滨海湿地根际微生物群落结构的影响研究[D];中国海洋大学;2013年
，本文编号：2181355
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