煤中有机物的水解菌源性产甲烷特性实验研究

发布时间：2018-08-30 18:57

【摘要】：位于淮北平原地区的两淮煤田含煤地层及相邻地层的甲烷含量丰富,是我国著名的高瓦斯、高突出矿井分布区和煤层气开发潜力矿区。通过细菌基因测序技术检测已发现矿井深部涌水中含有一定量的产甲烷菌(Methane production bacteria,简称MPB)。本实验所用的水样取自淮北煤田任楼矿井下中六采区运输大巷内的放水钻孔,终孔层位于C31-3石炭系太原组含溶洞石灰岩层,作为菌源水样,选取煤样中部分大分子有机物作为实验体系中的碳源和能源物质,进行生物性产甲烷实验。实验研究结果表明淮北矿区的煤系地下水中微生物菌群可以将煤中一些有机物水解成分子量较小的有机物,体现在这些有机物被降解的同时,丙酸、乙酸乙酯、辛酸乙酯等小分子有机酸含量在试验过程中整体呈现增长趋势。其中的2-己基-1-癸醇、己二酸二异辛酯、亚油酸3个实验组发现甲烷气体的产生,表明这些有机物被充分水解后生成的小分子有机酸物质可以被产甲烷菌所利用进行产出甲烷。验证了煤田区生物性产气的可行性,并显示出煤中大分子有机物水解产气的基本途径。通过对矿井水样品的古菌基因组DNA提取、mcr A基因片段扩增和文库构建,及基因序列同源性对比,发现淮北矿区煤系地下水中存在Methanoregula、Methanobacterium、Methanomethylovorans、Methanoregula、Methanolobus、Methanomassiliioccus,显示了本区煤系地下水中含有丰富的产甲烷菌群,其中Methanomassiliicoccus目古菌的发现填补了天然煤系产甲烷菌群记录的空白。
[Abstract]:The coal bearing strata and adjacent strata of Lianghuai coalfield located in Huaibei Plain are rich in methane content, which is a famous distribution area of high gas, high outburst mine and potential mining area for coalbed methane development in China. Detection of methanogenic bacteria (Methane production bacteria, in deep mine water by bacterial gene sequencing technique has been found to be short for MPB). The water samples used in this experiment are taken from the drainage boreholes in the transport roadway in the middle and sixth mining area of Renlou Coal Mine in Huaibei Coal Field. The final hole layer is located in the limestone layer of the cavernous limestone in the Taiyuan formation of the C31-3 Carboniferous system, which is used as the water sample from the bacteria source. Some macromolecular organic compounds in coal samples were selected as carbon and energy sources in the experimental system to conduct biological methanogenic experiments. The experimental results show that the microbial flora in coal measure groundwater in Huaibei mining area can hydrolyze some organic matter in coal into organic compounds with lower molecular weight, which is reflected in the degradation of these organic substances, propionic acid, ethyl acetate, The content of small organic acids such as ethyl octanoate showed an overall increasing trend during the experiment. Three experimental groups of 2-hexyl-1-decanol, diisooctyl adipate and linoleic acid found methane gas production, which indicated that the organic acids produced by these organic compounds could be used by methanogenic bacteria to produce methane. The feasibility of biologic gas production in coal field was verified, and the basic way of hydrolysis of macromolecular organic matter in coal was shown. The genomic DNA extracted from mine water samples were amplified by amplification and library construction of MCR A gene, and the homology of gene sequence was compared. It is found that the presence of Methanoregula,Methanobacterium,Methanomethylovorans,Methanoregula,Methanolobus,Methanomassiliioccus, in the groundwater of coal measures in Huaibei mining area shows that there are abundant methanogenic bacteria in the groundwater of coal measures in this area, and the discovery of Methanomassiliicoccus ancient bacteria fills up the blank in the record of methanogenic bacteria in natural coal measures.
【学位授予单位】：合肥工业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q78;TD841

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