醋酸盐兼养培养下蛋白小球藻油脂积累的研究

发布时间：2019-01-24 15:23

【摘要】：以醋酸盐作为蛋白小球藻兼养生长中的有机碳源,采用尼罗红三维荧光-区域体积积分法测定中性脂含量,研究了蛋白小球藻生长过程中的中性脂积累规律,分析了兼养和自养条件下生物量产率、总脂产率以及油脂的脂肪酸组成差异.结果显示,加入醋酸钠后,蛋白小球藻即进入对数增长期,生长迅速,且同时启动中性脂的积累.5g/L醋酸钠既能促使藻细胞生长和中性脂的持续积累,又能避免过高的有机碳源所带来的环境胁迫,培养12d后,单位细胞中性脂含量达到自养条件下对照样的4.5倍,生物量和总脂产率分别是对照样的2.1倍和3.1倍.5g/L醋酸钠作用下,油脂脂肪酸以饱和脂肪酸C16:0(22.42%)和不饱和脂肪酸C18:1(31.58%)、C18:2(10.89%)为主.
[Abstract]:Using acetate as the organic carbon source of Chlorella protein Chlorella, the neutral lipid accumulation in the growth process of Chlorella protein Chlorella was studied by means of the method of three dimensional fluorescence and regional volume integration of Nilored, which was used to determine the content of neutral lipid in the growth process of Chlorella vulgaris. The biological yield, total fat yield and fatty acid composition were analyzed. The results showed that when sodium acetate was added, Chlorella protein Chlorella entered the logarithmic growth period, grew rapidly and initiated the accumulation of neutral lipid at the same time. Sodium 5g/L acetate could not only promote the growth of algal cells and the accumulation of neutral lipid, but also promote the accumulation of neutral lipid. After being cultured for 12 days, the content of neutral lipid per unit cell was 4.5 times higher than that under autotrophic condition. The biomass and total fat yield were 2.1 times and 3.1 times higher than those of the control, respectively. Under the action of 5g/L sodium acetate, the fatty acids of oil and fat were C 16: 0 (22.42%) and C 18: 1 (31.58%), respectively. C18: 2 (10.89%).
【作者单位】：北京林业大学环境科学与工程学院北京市水体污染源控制技术重点实验室;
【基金】：中央高校基本科研业务费专项资金资助项目(2015ZCQ-HJ-02) 北京林业大学科学研究项目(2015PY-08) 教育部留学回国人员科研启动基金
【分类号】：TE667

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