全场高分辨生物组织光学层析成像
发布时间：2018-09-17 06:34
【摘要】：建立了一套全场光学相干层析(FFOCT)系统,以实现对生物组织和细胞的高分辨层析成像。该光学系统基于Linnik干涉显微结构。不同于现有FFOCT系统采用光纤束照明方式,采用卤钨灯照明和大数值孔径显微物镜成像,压电陶瓷(PZT)移相,面阵电荷耦合器件(CCD)采集干涉信号,由5步移相算法获取层析图,最终合成三维图像。对整套系统的性能进行了详细的阐述与分析,并通过对洋葱表皮细胞和盆栽树叶的光学层析实验,验证了本系统的可行性和精确度。通过对集成电路内部芯片(英特尔奔腾4,横向分辨率达0.8μm)成像,证明了该系统的分辨率可达到0.7μm×0.5μm(横向×纵向)。提出的系统分辨率高、成本低、结构简单便于调节,为实现高分辨率光学相干层析成像提供了简单易行的方法。
[Abstract]:A set of full-field optical coherence tomography (FFOCT) system was established to realize high resolution tomography of biological tissues and cells. The optical system is based on Linnik interference microstructure. Different from the existing FFOCT system, the optical fiber beam illumination is used, the Halogen-tungsten lamp illumination and the large numerical aperture microscopic objective imaging are used, the piezoelectric ceramic (PZT) phase shift, the plane charge coupled device (CCD) to collect the interference signal, and the five-step phase shift algorithm to obtain the chromatographic image. Finally, three-dimensional images are synthesized. The performance of the whole system was described and analyzed in detail. The feasibility and accuracy of the system were verified by optical chromatography of onion epidermis cells and potted leaves. By imaging the integrated circuit internal chip (Intel Pentium 4, lateral resolution 0.8 渭 m), it is proved that the resolution of the system can reach 0.7 渭 m 脳 0.5 渭 m (lateral 脳 longitudinal). The proposed system has the advantages of high resolution, low cost, simple structure and easy adjustment, which provides a simple and practical method for the realization of high resolution optical coherence tomography.
【作者单位】： 南京理工大学电光学院光学工程系;
【基金】：国家自然科学基金(61275198、60978069) 国防基础科研资助项目
【分类号】：TN911.7
本文编号：2245014
[Abstract]:A set of full-field optical coherence tomography (FFOCT) system was established to realize high resolution tomography of biological tissues and cells. The optical system is based on Linnik interference microstructure. Different from the existing FFOCT system, the optical fiber beam illumination is used, the Halogen-tungsten lamp illumination and the large numerical aperture microscopic objective imaging are used, the piezoelectric ceramic (PZT) phase shift, the plane charge coupled device (CCD) to collect the interference signal, and the five-step phase shift algorithm to obtain the chromatographic image. Finally, three-dimensional images are synthesized. The performance of the whole system was described and analyzed in detail. The feasibility and accuracy of the system were verified by optical chromatography of onion epidermis cells and potted leaves. By imaging the integrated circuit internal chip (Intel Pentium 4, lateral resolution 0.8 渭 m), it is proved that the resolution of the system can reach 0.7 渭 m 脳 0.5 渭 m (lateral 脳 longitudinal). The proposed system has the advantages of high resolution, low cost, simple structure and easy adjustment, which provides a simple and practical method for the realization of high resolution optical coherence tomography.
【作者单位】： 南京理工大学电光学院光学工程系;
【基金】：国家自然科学基金(61275198、60978069) 国防基础科研资助项目
【分类号】：TN911.7
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1 赵会娟,高峰,山田幸生;基于多通道时间分辨光学层析成像系统的差分图像重建[J];光学学报;2001年12期
，本文编号：2245014
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