Structured illumination microscopy for super-resolution and
本文关键词：Structured illumination microscopy for super-resolution and optical sectioning
更多相关文章： Microscopy Structured illumination Super-resolution Optical sectioning
【摘要】：Optical microscopy plays an essential role in biological studies due to its capability and compatibility of non-contact,minimally invasive observation and measurement of live specimens.However,the conventional optical microscopy only has a spatial resolution about200 nm due to the Abbe diffraction limit,and also lacks the ability of three-dimensional imaging.Super-resolution farfield optical microscopy based on special illumination schemes has been dramatically developed over the last decade.Among them,only the structured illumination microscopy(SIM)is of wide-field geometry that enables it easily compatible with the conventional optical microscope.In this article,the principle of SIM was introduced in terms of point spread function(PSF)and optical transform function(OTF)of the optical system.The SIM for super-resolution(SIM-SR)proposed by Gustafsson et al.and the SIM for optical sectioning(SIM-OS)proposed by Neil et al.are the most popular ones in the research community of microscopy.They have the same optical configuration,but with different data postprocessing algorithms.We mathematically described the basic theories for both of the SIMs,respectively,and presented some numerical simulations to show the effects of super-resolution and optical sectioning.Various approaches to generation of structured illumination patterns were reviewed.As an example,a SIM system based on DMDmodulation and LED-illumination was demonstrated.A lateral resolution of 90 nm was achieved with gold nanoparticles.The optical sectioning capability of the microscope was demonstrated with Golgi-stained mouse brain neurons,and the sectioning strength of 930 nm was obtained.
【作者单位】： State
【关键词】： Microscopy Structured illumination Super-resolution Optical sectioning
【分类号】：TH742
【正文快照】： 1 Introduction1.1 Illumination for microscopyIllumination of the specimen is the most important variablein achieving high-quality images in microscopy.Ko¨hlerillumination was first introduced in 1893 by Ko¨hler[1]as amethod of providing the optimum spe
【参考文献】
中国期刊全文数据库 前2条
1 ;Ultrahigh spatiotemporal resolved spectroscopy[J];Science in China(Series G:Physics,Mechanics & Astronomy);2007年06期
2 ;Hacking the optical diffraction limit: Review on recent developments of fluorescence nanoscopy[J];Chinese Science Bulletin;2011年18期
【共引文献】
中国期刊全文数据库 前10条
1 李艳秋;;浸没式ArF光刻最新进展[J];电子工业专用设备;2006年03期
2 江洋;任斌;田中群;;有孔探针近场拉曼光谱和成像技术进展[J];光谱学与光谱分析;2008年05期
3 王同舟;谢丽萍;Haley Huang;黎新;汪汝亮;杨光;杜亚楠;黄国亮;;Label-free biomolecular imaging using scanning spectral interferometry[J];Chinese Optics Letters;2013年11期
4 李冠楠;谭庆昌;寇莹;张宇鹏;;一字线结构光三维测量模型的新型标定方法[J];光子学报;2013年11期
5 代祥俊;戴美玲;杨福俊;卢位昌;姚亚卿;;基于面内位移测量和刚体旋转的形貌测量方法[J];东南大学学报(自然科学版);2013年06期
6 郝翔;匡翠方;李e，
本文编号：1130462
本文链接：https://www.wllwen.com/kejilunwen/yiqiyibiao/1130462.html
