智能便携式C-反应蛋白荧光检测系统

发布时间：2017-12-28 18:49

  本文关键词：智能便携式C-反应蛋白荧光检测系统　出处：《湖南师范大学》2016年硕士论文　论文类型：学位论文

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【摘要】：C-反应蛋白(C-reactive protein,CRP)是一种可与肺炎球菌细胞壁C-多糖反应,形成复合沉淀物的非抗体急性时相蛋白质,常常作为判断机体炎症状况的标记,广泛应用于临床医学检测中。近几十年国际医学界研究发现,CRP不仅反应机体炎症状况,也是心脏病、高血压、动脉粥样硬化等心血管类疾病的预警因子,定量检测其在血液中浓度,对预防和控制此类疾病有非常重要的实际意义。为了能准确、快速、简便地检测CRP浓度,本论文设计并实现了一种基于荧光检测的CRP免疫层析检测系统,该系统基于免疫标记技术、层析技术和荧光示踪检测技术,通过检测荧光标记荧光淬灭释放的荧光光强,结合光强-浓度定量模型,实现对目标CRP抗原浓度的定量检测。论文内容主要分以下几个部分:1、介绍CRP的检测背景意义及国内外的检测现状,通过对比常用的检测方法,提出本文采用的荧光检测方法,并阐述该方法的发展历史、发展现状和发展趋势。2、详细介绍本文荧光检测系统的工作原理,并阐述了系统的设计构架,包括检测平台组成、指标参数和影响因素。3、介绍检测系统的硬件设计,包括处理器资源分配,电源模块设计,USB扫描设备、激发光源设备及步进电机设备的驱动设计,信号调理电路设计、屏幕显示模块设计等。4、介绍系统的软件设计。首先介绍了软件系统的整体框架,然后描述了系统检测流程,接着以系统开机检测为主线,介绍了系统开机硬件初始化、USB条码扫描、电机驱动检测、结果保存等功能的软件设计,最后阐述软件实现系统定量检测的两个数据处理算法。5、对研制出来的检测仪进行多次试验分析。实验证明:该检测仪稳定性好、定量精准,RSD小于3%,最小可检测浓度为0.46mg/L,常规定量检测范围内,系统误差小于3%,符合临床医学检测需求。6、对全文进行了总结,并展望该检测仪发展改进的方向。
[Abstract]:C- C-reactive protein (CRP) is a non antibody acute phase protein that can react with pneumococcus cell wall C- polysaccharide to form complex precipitates. It is often used as a marker for judging the inflammatory state of the body, and is widely used in clinical medicine detection. Found in international medicine research in recent decades, CRP not only reflect the inflammatory status, predictors of heart disease, hypertension, atherosclerosis and other cardiovascular diseases, quantitative detection of its concentration in the blood, for the prevention and control has very important practical significance to this kind of disease. In order to accurately and quickly and easily detect the concentration of CRP, this paper designs and implements a CRP immune chromatography fluorescence detection detection system based on the immune system, labeling technique, chromatography and fluorescence tracer detection technique based on fluorescence quenching of fluorescence by detecting the fluorescence intensity of the release, combined with light concentration quantitative model and realize the quantitative detection of target CRP antigen concentration. The contents of the paper are mainly divided into the following parts: 1, introduce the background and significance of CRP detection and the detection status at home and abroad. By comparing the commonly used detection methods, we propose the fluorescence detection method adopted in this paper, and elaborate the development history, development status and development trend of this method. 2. The principle of the fluorescence detection system in this paper is introduced in detail, and the design framework of the system is described, including the composition of the detection platform, the parameters of the index and the influencing factors. 3, introduce the hardware design of the detection system, including processor resource allocation, power module design, USB scanning device, excitation source device and stepper motor drive design, signal conditioning circuit design, screen display module design, etc. 4. Introduce the software design of the system. First introduces the overall framework of the software system, and then describes the system testing process, and then to start testing system as the main line, introduces the software design of the system hardware boot initialization, USB bar code scanning, motor drive detection, save the results and other functions of the system, and finally realize the quantitative detection of the two data processing algorithm of software. 5. Test and analyze the developed detector many times. The experiment proved that the detector is stable and accurate. The RSD is less than 3%, and the minimum detectable concentration is 0.46mg/L. Within the conventional quantitative detection range, the system error is less than 3%, which accords with the needs of clinical medicine detection. 6. Summarize the full text and look forward to the direction of the development and improvement of the detector.
【学位授予单位】：湖南师范大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：TH773
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本文编号：1347002