基于可编程LED阵列照明的透射体视显微镜

发布时间：2018-06-17 12:12

本文选题：成像系统 + 显微镜　；参考：《光学学报》2016年05期

【摘要】：为了实现对待测样品高质量的体视显微成像,提出了基于可编程发光二极管(LED)阵列照明的透射体视显微镜。该显微镜光学系统使用可编程LED阵列作为照明双光源,照明孔径、角度以及波长均可自由选择,通过改变可编程LED阵列上红蓝圆形光源的半径r和圆心间距d分别实现对体视显微镜焦深和体视角的灵活可控。实验结果表明,该显微镜系统简便,观察者根据自身需要选择合适的焦深和体视角可有效解决因人眼瞳距个体差异大引起单一体视角出现的双像不能重合等问题,实现对待测样品的直接体视显微观看。使用数值孔径NA=0.1(4×)的显微物镜,在最佳参数r=2,d=3时可清晰观测到待测样品的层次关系以及相对位置等三维信息。
[Abstract]:In order to achieve high quality stereoscopic microscopic imaging of samples, a transmission stereoscopic microscope based on programmable light emitting diode (LED) array illumination was proposed. The optical system of the microscope uses programmable LED array as the double illumination light source. The aperture, angle and wavelength of the illumination can be chosen freely. By changing the radius r and the center distance d of the red and blue circular light source on the programmable LED array, the flexible control of the depth of focus and the angle of view of the stereoscopic microscope can be achieved respectively. The experimental results show that the microscope system is simple, and the suitable focal depth and body angle can be used to solve the problem of double image incoincidence caused by the individual difference of eye pupil distance. The direct stereoscopic microscopic view of the sample is realized. Using a microscopic objective with a numerical aperture of 0.1 ~ 4 脳), three dimensional information such as the hierarchical relationship and the relative position of the sample can be clearly observed at the optimum parameter r ~ (2) ~ (2) d = 3.
【作者单位】：南京理工大学电子工程与光电技术学院智能计算成像实验室;南京理工大学电子工程与光电技术学院江苏省光谱成像与智能感知重点实验室;
【基金】：中央高校基本科研业务费专项资金(30916011322) 国家自然科学基金(11574152;61505081) 江苏省"六大高峰"人才项目(2015-DZXX-009) 江苏省"333工程"科研项目资助计划(BRA2015294) 江苏省光谱成像智能感知重点实验室开放基金(3092014012200417)
【分类号】：TH742.2

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