基于石墨烯信号放大策略的胆固醇分子印迹电化学传感器研究

发布时间：2018-01-28 22:55

本文关键词： 石墨烯胆固醇分子印迹膜电化学传感器　出处：《分析化学》2017年10期 　论文类型：期刊论文

【摘要】：利用石墨烯的信号放大作用,结合分子印迹技术和电聚合技术,以苯酚为功能单体,胆固醇(ChO)为模板分子,在石墨烯修饰的玻碳电极表面电聚合能识别胆固醇分子的敏感膜,构建胆固醇分子印迹电化学传感器。采用扫描电镜、循环伏安法、差分脉冲伏安法研究了印迹膜的结构、性能和分子印迹效应。结果表明,此传感器对胆固醇有良好的选择性和较高的灵敏度。胆固醇浓度在8.0×10~(-8)~2.0×10~(-4)mol/L范围内与峰电流的变化呈线性关系,检出限(S/N=3)为5.6×10~(-8)mol/L。将其用于人体血清样品的检测,结果满意。
[Abstract]:By means of signal amplification of graphene, molecular imprinting and electropolymerization, phenol was used as functional monomer and cholesterol Cho as template molecule. The electropolymerization on the surface of graphene modified glassy carbon electrode can recognize the sensitive membrane of cholesterol molecule and construct the cholesterol molecular imprinted electrochemical sensor. Scanning electron microscope and cyclic voltammetry were used. The structure, properties and molecular imprinting effect of the imprinted membrane were studied by differential pulse voltammetry. The sensor has good selectivity and high sensitivity to cholesterol. The cholesterol concentration is 2.0 脳 10 ~ (-4). The range of mol/L is linear with the change of peak current. The detection limit is 5.6 脳 10 ~ (-1) ~ (-8) mol 路L ~ (-1). It has been applied to the detection of human serum samples with satisfactory results.
【作者单位】：云南大学化学科学与工程学院;云南大学材料科学与工程学院云南省微纳材料与技术重点实验室;云南省烟草质量监督检测站;
【基金】：云南省教育厅科学基金(No.2017ZZX231) 国家自然科学基金项目(Nos.21465025,21505119)资助~~
【分类号】：O657.1;TP212
【正文快照】： 1引言胆固醇(Cholesterol,Ch O)也称胆甾醇,是多种疾病诊断的重要生物指标[1]。血清中高胆固醇的存在是引发多种疾病(如冠心病、心肌梗塞、动脉硬化高血压等)的主要因素,而胆固醇低也可能会导致低脂蛋白血症、贫血、败血症、营养不良、甲亢和肝病[2]。因此,准确、灵敏、快速地

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