光致清洁电极可见光在线更新与细胞实时监测

发布时间：2018-02-20 18:18

本文关键词： 纳米光催化剂光致清洁电极可见光明胶人脐静脉内皮细胞一氧化氮实时监测　出处：《化学学报》2017年11期 　论文类型：期刊论文

【摘要】：电化学传感器在用于细胞实时监测过程中,电极界面污染严重影响其检测性能.通过将纳米光催化剂与电化学传感材料复合,构建光致清洁电化学传感器,为电极界面的高效及无损更新提供了新思路.然而光催化产生的活性氧自由基导致细胞损伤,限制了细胞培养及检测过程中电极界面的实时更新.为此,我们在PEDOT@CdS/TiO_2/ITO可见光致更新电极表面旋涂明胶薄层,在保持电极良好的光致清洁和电化学传感性能同时,利用明胶薄层阻碍光催化产生的活性氧自由基扩散至细胞表面,显著降低了细胞损伤.此外,明胶优良的生物相容性有利于细胞的黏附及增殖.利用该电极,我们实现了人脐静脉内皮细胞(HUVECs)培养过程中,电极的在线更新以及细胞释放一氧化氮的实时监测.
[Abstract]:In the process of cell real-time monitoring, electrode interface contamination seriously affects the performance of electrochemical sensor. By combining nano-photocatalyst with electrochemical sensor material, a photo-induced clean electrochemical sensor is constructed. It provides a new idea for high efficiency and nondestructive renewal of electrode interface. However, reactive oxygen free radicals produced by photocatalysis cause cell damage and limit the real-time updating of electrode interface during cell culture and detection. We spin-coated gelatin thin layer on the surface of the PEDOT@CdS/TiO_2/ITO visible light-induced renewal electrode. We used the gelatin thin layer to prevent the photocatalytic diffusion of reactive oxygen free radicals to the cell surface, while maintaining the good photo-induced cleanliness and electrochemical sensing performance of the electrode. In addition, the excellent biocompatibility of gelatin was beneficial to cell adhesion and proliferation. Online updating of electrodes and real-time monitoring of nitric oxide release from cells.
【作者单位】：生物医学分析化学教育部重点实验室武汉大学化学与分子科学学院;
【基金】：国家自然科学基金(Nos.21375099,21675121)资助~~
【分类号】：O657.1;TP212

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