基于MOCPs-MWCNTs的大肠杆菌电化学免疫传感器

发布时间：2018-03-05 09:43

本文选题：大肠杆菌O　切入点：H　出处：《农业机械学报》2017年06期 　论文类型：期刊论文

【摘要】：食源致病菌引起的食品安全问题已成为全世界关注的焦点。设计了一种基于多壁碳纳米管(MWCNTs)-有机配位聚合物(MOCPs)高效固定抗体的大肠杆菌电化学免疫传感器。利用NaAuCl_4与1,4-苯二硫醇(BDT)反应生成金属有机配位聚合物,同时在BDT的还原作用下生成大量纳米金,并包埋大量吸附在MWCNTs表面的大肠杆菌抗体,最终将抗体高效包埋在MOCPs-MWCNTs中,基于MOCPs-MWCNTs固定抗体研制修饰电极,在目标物大肠杆菌O157:H7的存在下,辣根过氧化氢酶标记的抗体(HRP-Ab)随后特异性捕获于电极表面形成典型夹心结构,通过HRP催化底物产生电化学信号从而实现大肠杆菌O157:H7的检测。MOCPs-MWCNTs特有的高比表面积高效固定了大量抗体,增加了传感器结合抗体的效率并提高了传感器的灵敏度。该传感器对大肠杆菌O157:H7的检测线性范围为67~6.7×10~6cfu/mL,最低检出限为40 cfu/mL。
[Abstract]:Food safety problems caused by food pathogenic bacteria have become the focus of worldwide attention. A novel electrochemical immunosensor for Escherichia coli based on multiwalled carbon nanotube MWCNTs-organic coordination polymer (MOCPs) was designed. A metal-organic coordination polymer was synthesized by the reaction of NaAuCl_4 with 1-diphenyl dimercaptan (BDT). At the same time, a large amount of gold nanoparticles were produced by the reduction of BDT, and a large number of Escherichia coli antibodies adsorbed on the surface of MWCNTs were embedded. Finally, the antibodies were effectively embedded in MOCPs-MWCNTs, and the modified electrode was developed based on the immobilized antibody of MOCPs-MWCNTs. In the presence of the target E. coli O157: H7, HRP-Abs, a horseradish catalase labeled antibody, was then specifically trapped on the electrode surface to form a typical sandwich structure. The specific high specific surface area of E. coli O157: H7 was detected by the electrochemical signal generated by the substrate catalyzed by HRP, and a large number of antibodies were efficiently immobilized. The sensitivity of the sensor was improved by increasing the efficiency of antibody binding. The linear range of detection for E. coli O157: H7 was 676.7 脳 10 ~ (6) cfur 路mL ~ (-1), and the lowest detection limit was 40 cm ~ (-1) 路u ~ (-1) 路mol ~ (-1) 路L ~ (-1) 路L ~ (-1).
【作者单位】：浙江省农业科学院农产品质量标准研究所;
【基金】：浙江省自然科学基金项目(LZ15C170001)
【分类号】：TP212

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