核酸自动提取仪控制系统的研制

发布时间：2018-05-25 04:24

本文选题：分子诊断 + 核酸提取　；参考：《南京航空航天大学》2017年硕士论文

【摘要】：随着近年来分子技术的高速发展,分子诊断技术已经成为生物学最基本的技术之一,它在人体健康体检、物种鉴定等领域发挥着愈来愈重要的作用。分子诊断的主要技术包含核酸提取技术、核酸分子杂交以及核酸扩增技术,其首要任务便是核酸的提取,因此提高核酸提取的量和质,在分子诊断技术中具有重要的意义。本文根据核酸提取仪的功能要求和技术指标,按照模块化设计思想,提出了包括主控模块、智能LCD模块、温控模块的核酸提取仪总体方案设计;论文对以上各模块进行了主要元器件试验选型、设计了电路原理图和PCB图、制作了印刷线路板,完成了电路的静态和动态调试;在软件方面,采用JavaScript开发了可编程智能LCD模块的用户界面、通讯协议、图像显示等模块,按照嵌入式系统的特点,主MCU移植了Ucosii操作系统,开发了运动控制、数据存取及上下位机通讯模块的软件;根据系统温度要求,设计了一套温控系统,并能通过主MCU进行温度控制;在系统可靠性方面,论文对机器参数的整定、数据掉电保护、枪针遗漏检测等进行了设计,提高了核酸提取仪在使用过程中的可靠性。系统平台已经搭建完成,硬件和软件已经调试完毕,并进行了相应的参数优化。后期在系统平台上做了相关的精度和均匀性实验,充分验证了系统的可行性。
[Abstract]:With the rapid development of molecular technology in recent years, molecular diagnosis technology has become one of the most basic biological technology, it plays an increasingly important role in the field of human health examination, species identification and so on. The main techniques of molecular diagnosis include nucleic acid extraction, nucleic acid hybridization and nucleic acid amplification. The most important task is to extract nucleic acid, so it is of great significance to improve the quantity and quality of nucleic acid extraction. According to the function requirement and technical index of nucleic acid extractor, according to the modular design idea, this paper puts forward the overall scheme design of nucleic acid extractor including main control module, intelligent LCD module and temperature control module. In this paper, the main components of the above modules are selected, the circuit schematic diagram and PCB diagram are designed, the printed circuit board is made, and the static and dynamic debugging of the circuit is completed. The user interface, communication protocol and image display module of programmable intelligent LCD module are developed by using JavaScript. According to the characteristics of embedded system, the main MCU transplanted Ucosii operating system and developed motion control. According to the temperature requirement of the system, a set of temperature control system is designed, and the temperature control system can be controlled by the main MCU. In the aspect of system reliability, the paper sets the parameters of the machine and protects the data from power loss. Gun needle omission detection was designed to improve the reliability of nucleic acid extractor. The system platform has been built, the hardware and software have been debugged, and the corresponding parameters have been optimized. In the later stage, the accuracy and uniformity experiments were done on the system platform, which fully verified the feasibility of the system.
【学位授予单位】：南京航空航天大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q503;TP273

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