基于温度的酶注射式葡萄糖生物传感器检测方法研究

发布时间：2018-06-15 22:30

  本文选题：温度 + 葡萄糖　； 参考：《分析化学》2017年01期

【摘要】：针对酶注射式葡萄糖生物传感器在实际使用中因为标定液与被测液的温度不同而引起的测量结果不准确问题,提出一种基于温度的葡萄糖浓度检测方法。首先根据酶促反应动力学建立目前酶注射式葡萄糖生物传感器浓度检测模型,之后利用阿伦尼乌斯公式建立温度与浓度检测动力学模型中未知参数之间的关系,并将此关系代入浓度检测动力学模型中,以建立基于温度的浓度检测新模型。此模型以温度与酶促反应的电流初始斜率为输入值,以被测葡萄糖浓度为输出值,利用此模型提出了以反应混合液的温度和反应初始电流斜率推导被测液浓度的检测方法。利用改进的检测方法进行检测,不仅能够降低温差的影响,提高检测的准确性,还可以省略常规检测中的人工标定,避免人工标定所需的取样探头拆卸步骤,更加有利于在线使用。分别在25.0,30.0和42.0℃下检测1.5 mg/m L和2.5 mg/m L葡萄糖溶液,利用原检测方法与基于温度的检测方法进行检测,结果表明,基于温度的检测方法回收率均在95.0%以上,明显优于原检测方法。
[Abstract]:In view of the inaccuracy of the measurement results caused by the temperature difference between the calibrated solution and the measured liquid in the practical use of the enzyme injection glucose biosensor, a temperature based glucose concentration detection method is proposed. According to the kinetics of enzymatic reaction, the current concentration detection model of enzyme injection glucose biosensor was established, and then the relationship between temperature and unknown parameters in the kinetic model of concentration detection was established by using Arrhenius formula. A new concentration detection model based on temperature was established by inserting the relationship into the concentration detection kinetic model. In this model, the initial current slope of the enzymatic reaction is taken as the input value and the glucose concentration measured as the output value. Based on this model, a method for deriving the measured concentration from the temperature of the reaction mixture and the initial current slope of the reaction solution is proposed. The improved detection method can not only reduce the influence of temperature difference and improve the accuracy of detection, but also omit the manual calibration in the routine test, and avoid the sampling probe disassembly steps needed for manual calibration. More conducive to online use. 1. 5 mg/m / L and 2. 5 mg/m / L glucose solutions were detected at 25.0 and 42. 0 鈩，

本文编号：2023884