便携式船舶压载水中微藻检测系统设计

发布时间：2018-10-16 14:00

【摘要】：随着船舶压载水的给排过程,船舶压载水中携带的细菌、微藻等生物被排放到新的海域,这是造成海洋外来生物入侵的主要途径,且已经严重威胁了海洋的生态环境。快速准确的检测出船舶压载水中含有的微藻对船舶压载水的处理和海洋生态系统的保护都有重要的意义。目前已有的船舶压载水中微藻检测技术还无法做到方便快速和现场实时检测。针对现有技术存在的问题,本文提出了一种方便快速的检测方法和便携式船舶压载水中微藻检测系统。该系统的检测原理是利用侧向散射光检测技术、激光诱导荧光检测技术和阻抗脉冲传感检测技术分别对微藻的粒度、叶绿素荧光和粒径进行检测,并通过对三种信号的分析实现对船舶压载水中微藻的检测,同时利用微流控芯片作为三种检测技术的集成检测平台,有效的减小了检测系统的体积。整个检测系统由微流控芯片模块、信号检测模块、信号处理模块、ARM控制模块、电源模块、泵驱动模块和机械结构模块等组成,检测设备的体积小,易于携带。为了验证系统的性能,首先对各分模块和集成后的系统进行了验证性测试,然后取三种典型的微藻样品进行实际检测,并将检测结果与标准流式细胞仪的检测结果进行对比,通过对比证明本系统的准确性。本文提出的检测系统与常用的微藻检测设备相比,具有检测快速、耗样少、便携等优点。
[Abstract]:With the process of ballast water supply and discharge, the bacteria, microalgae and other organisms carried in the ballast water are discharged into the new sea area, which is the main way to cause the invasion of marine alien organisms, and has seriously threatened the marine ecological environment. The rapid and accurate detection of microalgae in ship ballast water is of great significance to the treatment of ship ballast water and the protection of marine ecosystem. At present, the existing microalgae detection technology in ballast water of ships is not convenient and fast and can not be detected in real time. In this paper, a convenient and rapid detection method and a portable detection system for microalgae in ballast water of ships are presented. The detection principle of the system is to detect the particle size, chlorophyll fluorescence and particle size of microalgae by using lateral scattering light detection technology, laser induced fluorescence detection technique and impedance pulse sensing technology, respectively. And through the analysis of three signals to achieve the detection of marine ballast water microalgae, at the same time, using microfluidic chip as the integrated detection platform of the three detection technologies, effectively reduce the volume of the detection system. The whole detection system is composed of microfluidic chip module, signal detection module, signal processing module, ARM control module, power supply module, pump driving module and mechanical structure module. In order to verify the performance of the system, the verification tests were carried out on the sub-modules and the integrated system, and then three typical microalgae samples were taken for actual detection, and the results were compared with those of the standard flow cytometry. The accuracy of the system is proved by comparison. Compared with the commonly used microalgae detection equipment, the detection system presented in this paper has the advantages of fast detection, less sample consumption and portability.
【学位授予单位】：大连海事大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：U664.92;TP274

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