基于荧光纳米粒子的生物传感器的制备及应用

发布时间：2018-11-16 18:26

【摘要】：上转换纳米颗粒具有高穿透性、无细胞组织损伤、高的信噪比以及良好的生物相溶性等优点,因此在生物荧光标记成像、生物检测及药物治疗领域具有巨大的应用价值,是目前国际研究的热点。本文主要以基于荧光纳米粒子的生物传感器为研究对象,制备了高精度生物传感器,并基于该生物传感器对植物细胞成像、生物体残留的罗丹明B有机染料和糖尿病患者血液中提取的糖化血红蛋白进行定量的检测。论文的主要内容如下:(1)实现了上转换纳米颗粒的制备。上转换纳米颗粒是制备生物传感器的核心材料之一,通过溶剂热法合成尺寸均匀,发光强度高的上转换纳米颗粒。该上转换纳米颗粒在980nm的近红外光激发下,发出耀眼的可见光。(2)使用生物传感器进行细胞成像。相比于传统的基于透射光的光学成像,应用上转换纳米探针对植物活体进行荧光显微成像克服了对细胞切片的依赖,能无损害无创伤进行活体成像。基于差异化成像原理,有望用于人体病变组织的精确定位,为进一步的病变组织治疗提供必备的基础。(3)荧光纳米粒子生物传感器对有机染料进行定量检测。基于荧光共振能量传递基本原理,构建了传感体系,计算上转换发光光谱的红绿积分强度比,从而构建红绿积分强度比与罗丹明B浓度的函数模型,求出积分强度比值后找到对应的罗丹明B浓度,从而实现罗丹明B的定量检测。(4)阵列型生物传感器用于糖化血红蛋白的定量检测和分析。阵列型生物传感器能够有效的用于检测不同浓度的糖化血红蛋白溶液。由于上转换阵列传感器薄膜上存在消光效应,使用其检测糖化血红蛋白溶液时具有更高的检测精度。该荧光阵列传感器用于检测糖化血红蛋白具有更高的检测精度(是在溶液中检测精度的4倍),更优的荧光稳定度(误差在0.4%~1.8%)和很好的可重复性(重复使用20次而不存在偏差)。
[Abstract]:The up-conversion nanoparticles have the advantages of high penetration, no cellular tissue damage, high signal-to-noise ratio and good biocompatibility, so they have great application value in the field of bioluminescent imaging, biological detection and drug therapy. Is the current international research hot spot. In this paper, the biosensor based on fluorescent nanoparticles was used as the research object, and the high precision biosensor was fabricated, and based on the biosensor, the plant cells were imaged. Residual Rhodamine B organic dyes and glycosylated hemoglobin extracted from the blood of diabetic patients were detected quantitatively. The main contents of this paper are as follows: (1) the preparation of up-conversion nanoparticles is realized. Up-conversion nanoparticles are one of the core materials for biosensors. The up-conversion nanoparticles with uniform size and high luminescence intensity are synthesized by solvothermal method. The up-converted nanoparticles emit dazzling visible light excited by the near-infrared light of 980nm. (2) Cell imaging using biosensors. Compared with the traditional optical imaging based on transmission light, fluorescence microimaging with up-conversion nanoprobes can overcome the dependence on cell sections and can be used in vivo imaging without damage and trauma. Based on the principle of differential imaging, it is expected to be used in the accurate localization of human pathological tissues, and provide an essential basis for further treatment of pathological tissues. (3) fluorescent nanoparticles biosensor quantitative detection of organic dyes. Based on the fundamental principle of fluorescence resonance energy transfer, the sensing system is constructed, and the red-green integral intensity ratio of up-conversion luminescence spectrum is calculated, and the function model of the ratio of red and green integral intensity to the concentration of rhodamine B is constructed. The corresponding Rhodamine B concentration was found after the integral intensity ratio was calculated, and the quantitative detection of Rhodamine B was realized. (4) the array biosensor was used for quantitative detection and analysis of glycosylated hemoglobin. Array biosensors can be used to detect different concentrations of glycosylated hemoglobin solution. Because of the extinction effect on the film of up-conversion array sensor, the detection accuracy of saccharified hemoglobin solution is higher when it is used. The fluorescent array sensor is used to detect glycosylated hemoglobin with higher accuracy (four times more accurate than in solution). Better fluorescence stability (error of 0.4% or 1.8%) and good repeatability (repeated 20 times without deviation).
【学位授予单位】：湖南科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TP212.3

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