NaOH蚀刻玻碳电极的大肠杆菌DNA电化学生物传感器的构建及检测
发布时间：2019-06-08 11:56
【摘要】：以NaOH蚀刻后的玻碳电极为基底制备了高灵敏与高选择性的大肠杆菌电化学DNA传感器。经NaOH蚀刻后的玻碳电极被活化且在电极表面形成羧基层,为DNA探针的固定提供了更多位点,在偶联剂EDC/NHS的作用下,端氨修饰的探针DNA与羧基的羧氨反应使其以肽键的形式固定在电极表面,极大地提升了传感器的灵敏度。通过电化学循环伏安法(CV)和差分脉冲法(DPV)对所制备传感器的灵敏度和选择性进行表征,得到该传感器对大肠杆菌的线性检测范围为12.5~62.5 nmol/L,检出限可达1.20×10~(-9)mol/L。
[Abstract]:A highly sensitive and selective E. coli electrochemical DNA sensor was prepared on the substrate of NaOH etched glassy carbon electrode. The glassy carbon electrode etched by NaOH is activated and forms a carboxy base on the electrode surface, which provides more sites for the fixation of DNA probe. Under the action of coupling agent EDC/NHS, The reaction of ammonia-terminated probe DNA with carboxy group makes it fixed on the electrode surface in the form of peptide bond, which greatly improves the sensitivity of the sensor. The sensitivity and selectivity of the sensor were characterized by electrochemical cyclic Voltammetric method (CV) and differential pulse method (DPV). The linear detection range of the sensor for E. coli was 12.5 鈮，
本文编号：2495265
[Abstract]:A highly sensitive and selective E. coli electrochemical DNA sensor was prepared on the substrate of NaOH etched glassy carbon electrode. The glassy carbon electrode etched by NaOH is activated and forms a carboxy base on the electrode surface, which provides more sites for the fixation of DNA probe. Under the action of coupling agent EDC/NHS, The reaction of ammonia-terminated probe DNA with carboxy group makes it fixed on the electrode surface in the form of peptide bond, which greatly improves the sensitivity of the sensor. The sensitivity and selectivity of the sensor were characterized by electrochemical cyclic Voltammetric method (CV) and differential pulse method (DPV). The linear detection range of the sensor for E. coli was 12.5 鈮，
本文编号：2495265
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