刺激响应荧光材料在生物传感的应用

发布时间：2021-01-10 01:23

　　荧光是生物成像和生物传感中最通用和广泛使用的技术之一,因为它可以原位研究生物样品,并在分子水平上提供非常合适的证据,具有较高的时间和空间分辨率。然而,将荧光材料用于生物传感中,还存在着低水溶性、较强的细胞毒性、合成困难、繁琐的聚合物修饰和较低的量子产率等问题。最近,荧光染料和具有非常规发色团的聚合物越来越受到人们的重视。而有机小分子荧光染料由于具有易于改性的结构、尺寸小和容易调节性能等性质,越发受到人们的关注。类似地,具有非常规发色团的刺激响应性荧光聚合物也因其良好的生物相容性,较好的水溶性和优异的稳定性而备受关注。本文讨论了刺激响应性、生物相容性的荧光染料和聚合物在生物传感中的应用。我们的工作可分为两个部分。在第一部分也是最主要部分中,报道了聚乙二醇（PEG）修饰的基于胱胺的多功能荧光染料的合成与表征以及其在检测F-和Cu2+中的应用。我们采用一锅法合成了CPEG-168、CPEG-1000和CPEG-2000荧光染料,在纯水性介质中量子产率取决于链长。由于含有二硫化物基团,这些化合物很可能能检测到硫醇的存在。链长-NH基团的高螯合亲和力获得了对Cu2+离子的刺激响应性,而pH和F-... 

【文章来源】：中国科学技术大学安徽省 211工程院校 985工程院校

【文章页数】：144 页

【学位级别】：博士

【文章目录】：
摘要
ABSTRACT
ACKNOWLEDGEMENT
Chapter 1. Introduction
    1.1 Motivation and general background
    1.2 Fluorescent compounds with conventional chromophores
        1.2.1 Fluorophores based on oxygen heterocycles
        1.2.2 Fluorophores based on sulfur heterocycles
        1.2.3 Fluorophores based on nitrogen heterocycles
        1.2.4 Naphthalene Fluorophores
    1.3 Fluorescent polymers with conventional chromophores
        1.3.1. Polyfluorenes （PFs） based CPs
        1.3.2. Poly（p-phenyleneethynylene） （PPE） based CPs
        1.3.3. Polythiophene based CPs
        1.3.4. Polydiacetylenes （PDA） based CPs
        1.3.5. Conjugated dendrimer-based sensors
    1.4. Fluorescent compounds with unconventional chromophores
    1.5. Fluorescent polymers with unconventional chromophores
    1.6. pH sensitive fluorescent materials
        1.6.1. pH sensitive probes based rhodamine
        1.6.2. pH probes based on phenols
        1.6.3. pH probes based on protonation/deprotonation of N-heterocycles
        1.6.4. pH probes based on protonation/deprotonatio amines
        1.6.5. pH probes based on dendrimers
    References
Chapter 2. Fluorescent Materials for Detection of Ions and Thiols
    2.1. Fluorescent chemosensors for fluoride ions
        2.1.1. Importance of fluoride ions
        2.1.2. Fluorophores used for the development of probes for fluoride ions
        2.1.3. Fluorescent probes for fluoride ions based on hydrogen bonding
        2.1.4. Recent developments in fluorescent probes for fluoride ions
    2.2. Fluorescent chemosensors for copper （Ⅱ） ions
        2.2.1. Importance of copper （Ⅱ） ions
2+ ions">        2.2.2. Fluorophores used for the development of probes for Cu²⁺ ions
        2.2.3. Recent developments in fluorescent probes for copper （Ⅱ） ions
    2.3. Fluorescent chemosensors for thiols
        2.3.1. Biological importance of thiols
        2.3.2. Functionalities used to develop optical probes for thiols
        2.3.3. Recent developments in fluorescent probes for thiols
        2.3.4. Fluorescent probes based on disulfide-cleavage by thiols
    2.4. Aims and objectives
        2.4.1. Problem statement
        2.4.2 Aims
        2.4.3 Objectives
    References
Chapter 3. Polyethylene Glycol Modified Cystamine for Fluorescent Sensing
    3.1. Introduction
    3.2. Materials and methods
    3.3. Instrumentation
    3.4. Synthesis of CPEGs
    3.5. Results and Discussion
-, Cu²⁺)">    3.6. Application of fluorescent Chemosensor for detections of ions (F^-, Cu²⁺)
    3.7. Conclusion
    References
Chapter 4. Stimuli Responsive Hyperbranched Poly（amido amine）s for FluorescentSensing
    4.1. Introduction
    4.2. Materials and methods
    4.3. Instrumentation
    4.4. Syntheses of other poly（amido amine）s
        4.4.1 Synthesis of N,N'-cystaminebisacrylamide （CBA） monomer
        4.4.2 Synthesis of hyperbranched poly（amido amine）s （PAMAM） with andwithout disulfide group
        4.4.3. Disulfide-cleavage of PAMAM by DTT
    4.5. Results and discussion
    4.6. Conclusion
    References
Chapter 5. General Conclusions and Future Perspectives
    5.1. Conclusions
    5.2. Future perspective
List of Publications



本文编号：2967783