间充质干细胞对内毒素诱导急性肾损伤的作用

发布时间：2017-12-28 14:19

本文关键词：间充质干细胞对内毒素诱导急性肾损伤的作用　出处：《暨南大学学报(自然科学与医学版)》2017年03期 　论文类型：期刊论文

【摘要】：目的:脓毒症急性肾损伤(AKI)是一个严重而常见的疾病,目前尚无具体的治疗策略.本研究拟探讨脐带间充质干细胞(MSC)对内毒素诱导的AKI的作用.方法:腹腔注射LPS(质量分数为10 mg/kg)诱导AKI模型,30min后尾静脉注射不同剂量的MSC.1周后处死动物,收集肾组织和血清标本进行检测,并且评估大鼠的生存状况.结果:LPS能引起全身性炎症反应,促炎因子水平增加在脓毒症的发病中具有重要的作用.MSC治疗1周后,大鼠血清和肾组织中的TNF-α和IL-1β显著下降,IL-10显著上升(P0.05).此外,MSC能提高大鼠生存率,降低肾损伤.结论:静脉注射MSC,对LPS诱导的大鼠AKI具有治疗作用.MSC移植可以改善肾脏病理,减轻炎症反应,提高AKI大鼠的生存率.
[Abstract]:Objective: sepsis induced acute kidney injury (AKI) is a serious and common disease, there is no specific treatment strategies. This study intends to explore the umbilical cord mesenchymal stem cells (MSC) on endotoxin induced AKI. Methods: the intraperitoneal injection of LPS (mass fraction of 10 mg/kg) induced by AKI model 30min, after intravenous injection of different doses of MSC.1 weeks after the death of animal, collection of renal tissue and serum samples were detected, and the evaluation of rat survival. Results: LPS induced systemic inflammatory response, increased levels of proinflammatory cytokines in the pathogenesis of sepsis has an important role for.MSC after 1 weeks of treatment, serum and renal tissues of rats with TNF- alpha and IL-1 beta decreased significantly, IL-10 increased significantly (P0.05). In addition, MSC can improve the survival rate of rats, reduce the renal injury. Conclusion: intravenous injection of MSC, has the therapeutic effect of.MSC transplantation can improve renal pathology in LPS rats induced by AKI, Reduce the inflammatory response and improve the survival rate of AKI rats.
【作者单位】：深圳市人民医院肾内科&深圳市肾脏病重点实验室;深圳市人民医院血液内科;
【基金】：广东省科技厅基础项目(2014A020212472) 广东省深圳市卫生计生科研项目(201505002)
【分类号】：R459.7;R692
【正文快照】： 在临床上,脓毒症休克常引起肾功能损伤,肾脏是脓毒症休克的重要靶器官[1].急性肾损伤发生率在一般脓毒症患者中占约19%;在重度脓毒症患者中占约为23%;在血培养阳性的脓毒症休克者中占比可高达51%;脓毒症并发AKI的病死率高达70%[2].脓毒症引起的肾损伤主要是由过度释放炎性介质

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