广西地区壮族和汉族血小板捐献者HLA基因多态性的初步分析

发布时间：2017-12-30 20:19

本文关键词：广西地区壮族和汉族血小板捐献者HLA基因多态性的初步分析　出处：《东南大学学报(医学版)》2017年05期 　论文类型：期刊论文

【摘要】：目的:建立广西地区壮族和汉族血小板供者人类白细胞抗原(HLA)基因分型资料库,分析其HLAA、B基因频率及单体型频率的分布,解决临床患者的血小板输注无效(PTR)。方法:采用聚合酶链反应-序列特异寡核苷酸探针杂交技术(PCR-SSOP)对广西地区172名壮族和403名汉族的单采血小板捐献者进行HLA-A、B基因分型。结果:广西地区壮族血小板捐献者HLA-A和HLA-B位点的基因频率都符合HardyWeinberg遗传平衡(P0.05),共检出11种HLA-A等位基因、24种HLA-B等位基因和58种单倍体型;广西地区汉族血小板捐献者HLA-A和HLA-B位点的基因频率都不符合Hardy-Weinberg遗传平衡(P0.05),共检出14种HLA-A等位基因、30种HLA-B等位基因和100种单倍体型。结论:广西地区壮族和汉族血小板供者HLA基因特点比较接近中国南方人群,但也有广西地区特点,该结果可以为PTR患者迅速寻找到HLA相合血小板提供帮助。
[Abstract]:Objective: to establish the HLA-HLA genotyping database of platelet donors from Zhuang and Han ethnic groups in Guangxi, and to analyze the distribution of HLA-Amb gene and haplotype frequency. Methods: polymerase chain reaction-sequence specific oligonucleotide probe hybridization (PCR-SSOP) was used to solve the problem of ineffective platelet transfusion in clinical patients. HLA-A was performed on 172 single platelet donors from Zhuang nationality and 403 Han nationality in Guangxi. Results: the frequencies of HLA-A and HLA-B loci in Zhuang blood platelet donors in Guangxi were in accordance with the HardyWeinberg genetic balance (P0.05). A total of 11 HLA-A alleles, 24 HLA-B alleles and 58 haploid types were identified. The gene frequencies of HLA-A and HLA-B loci in Guangxi Han blood platelet donors were not in accordance with the Hardy-Weinberg genetic balance (P0.05). A total of 14 HLA-A alleles were detected. 30 HLA-B alleles and 100 haploid types. Conclusion: the characteristics of HLA gene in Zhuang and Han platelet donors in Guangxi are similar to those in southern China, but also in Guangxi. The results may be helpful for PTR patients to find HLA compatible platelets quickly.
【作者单位】：南宁中心血站南宁输血医学研究所;
【基金】：南宁市科学研究与技术开发计划项目(201003048C-3)
【分类号】：R457.11
【正文快照】： 血小板携带的抗原可分为两大类,即血小板相关性抗原和血小板特异性抗原(HPA),其中人类白细胞抗原(HLA)-A、HLA-B及HPA在血小板输注中具有临床意义。血小板输注无效(PTR)被定义为至少2次ABO血型相合而保存时间少于72 h的血小板输注后,输注效果不满意。在多次输注血小板治疗的患

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