有氧运动和膳食干预改善代谢综合征大鼠脂质代谢的效果及过氧化物酶体增殖物激活受体α介导的机制

发布时间：2017-12-31 05:33

本文关键词：有氧运动和膳食干预改善代谢综合征大鼠脂质代谢的效果及过氧化物酶体增殖物激活受体α介导的机制　出处：《中国康复理论与实践》2017年06期 　论文类型：期刊论文

【摘要】：目的通过对代谢综合征大鼠施加运动和膳食干预,观察有氧运动对代谢综合征脂代谢紊乱的调节作用,并探讨过氧化物酶体增殖物激活受体α(PPARα)介导的可能机制。方法 Sprague-Dawley大鼠喂养1周后随机分为空白对照组(CC组)和造模组,后者高脂高盐饲料喂养18周。造模成功后,将成模大鼠随机分为模型对照组(MC组,n=9)、模型高脂运动组(MHE组,n=11)和模型普食运动组(ME组,n=11)。ME组和MHE组跑台训练12周后测定体质量、肾周脂质量、血游离脂肪酸(FFA)、血脂,用荧光定量RT-PCR和Western blotting法测定心肌组织中的PPARαm RNA和蛋白表达水平。结果与CC组相比,MC组体质量、肾周脂质量、FFA、血脂升高(P0.05),PPARαm RNA和蛋白表达水平均明显降低(P0.01);与MC组比较,MHE组和ME组体质量、肾周脂质量、血三酰甘油(TG)较均降低(P0.05),高密度脂蛋白(HDL)、PPARαm RNA和蛋白表达水平升高(P0.05);与MHE组比较,ME组低密度脂蛋白(LDL)水平降低(P0.05),PPARαm RNA和蛋白表达水平明显升高(P0.01)。结论有氧训练能激活PPARα的表达,加强脂肪酸的利用,从而降低代谢综合征大鼠体质量和内脏脂肪质量,调节机体脂代谢。
[Abstract]:Objective to observe the effect of aerobic exercise on lipid metabolism disorder in metabolic syndrome rats by exercise and dietary intervention. To explore the possible mechanism of peroxisome proliferator-activated receptor 伪 -PPAR 伪. Methods Sprague-Dawley rats were randomly divided into control group and CC group after feeding for 1 week. And modeling. After the rats were fed with high fat and high salt diet for 18 weeks, the rats were randomly divided into the model control group (MC group) and the model high fat exercise group (MHE group). After 12 weeks of treadmill training, body mass, perirenal fat, serum free fatty acids and blood lipids were measured. The expression of PPAR 伪 m RNA and protein in myocardial tissue was measured by fluorescence quantitative RT-PCR and Western blotting. The results were compared with those of CC group. The expression of PPAR 伪 m RNA and protein in MC group, renal pericarpium weight and serum lipid level were significantly decreased (P 0.01). Compared with MC group, MHE group and ME group were significantly lower than those in MHE group and ME group in body weight, perirenal lipid mass, triglyceride TGG, HDL. The expression of PPAR 伪 m RNA and protein increased in P0.05; Compared with MHE group, low density lipoprotein (LDL-) level in ME group was lower than that in MHE group (P 0.05). The expression of PPAR 伪 m RNA and protein increased significantly (P 0.01). Conclusion aerobic training can activate the expression of PPAR 伪 and enhance the utilization of fatty acids. Therefore, the body weight and visceral fat mass of metabolic syndrome rats were decreased and lipid metabolism was regulated.
【作者单位】：井冈山大学体育学院;
【分类号】：R493;R589
【正文快照】： 随着社会经济的发展和生活方式的改变,高血质量、腹围,尾静脉取血测量空腹血糖、血脂等指压、糖尿病、肥胖和血脂紊乱的发病率逐年升高,这标。参照2005年国际糖尿病联盟颁布的代谢综合征诊些慢性病相互影响,并常在同一个体共同存在,被统断标准[4],选择具备腹型肥胖(即体质量与

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