浙江省柯萨奇病毒A16 VP1基因特征分析及B细胞抗原表位预测

发布时间：2017-12-31 09:11

本文关键词：浙江省柯萨奇病毒A16 VP1基因特征分析及B细胞抗原表位预测　出处：《临床检验杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的了解浙江省柯萨奇病毒A16(CA16)VP1基因特征及其变迁规律并预测VP1蛋白上B细胞抗原表位。方法在Gen Bank检索并下载35个浙江省CA16流行株及69个已知基因型CA16参考株的VP1基因序列,使用MEGA 6.0软件进行比对分析和构建种系进化树,确定浙江省CA16流行株的基因型,并分析VP1核苷酸和氨基酸同源性及氨基酸变异;运用DNA Star中的Protean模块预测VP1上可能的线性B淋巴细胞抗原表位。结果浙江省CA16病毒基因型为B1基因型(34株)和A基因型(1株)。B1型中以B1b亚型(24株)占绝对优势,其次为B1a亚型(10株)。浙江省CA16流行株VP1核苷酸和氨基酸同源性分别为75.5%~100%和89.5%~100%;VP1区氨基酸序列高度保守,但也存在部分变异。VP1中可能的B细胞抗原表位(氨基酸肽段)有13个,分别为10-20、30-38、57-62、73-79、98-106、118-123、159-168、173-177、184-189、240-246、265-273、274-284和289-295。结论浙江省CA16流行株存在A、B1 2个基因型,B1b和B1a为主要基因型,其VP1区氨基酸变异程度较低,VP1中13个预测的B细胞抗原表位可为CA16疫苗的研制提供科学依据。
[Abstract]:Objective to understand Coxsackie virus A16 CA16 in Zhejiang Province. The characteristics of VP1 gene and its changes and the prediction of B cell epitopes on VP1 protein. The VP1 gene sequences of 35 CA16 epidemic strains and 69 known CA16 reference strains in Zhejiang Province were retrieved and downloaded by Bank. The MEGA 6.0 software was used to analyze and construct the phylogenetic tree, to determine the genotype of CA16 epidemic strains in Zhejiang Province, and to analyze the nucleotide and amino acid homology and amino acid variation of VP1. The Protean module of DNA Star was used to predict the possible linear B lymphocyte epitopes on VP1. Results the genotype of CA16 virus in Zhejiang Province was 34 strains with B1 genotype. B 1b subtype (24 strains) was the dominant genotype. The nucleotide and amino acid homology of VP1 of CA16 epidemic strains in Zhejiang Province were 75.555% and 89.5%, respectively. The amino acid sequence of VP1 region was highly conserved, but there were also some variations. There were 13 possible B cell epitopes (amino acid peptide) in VP1, which were 10-20 ~ 30-38 ~ 57-62, respectively. 73-79C98-106C 118-123C 159-168N 173-177-184-189C240-246C265-273. Conclusion there are two genotypes of CA16 in Zhejiang Province, B 1b and B 1a, and the degree of amino acid variation in VP1 region is low. The 13 predicted B cell epitopes in VP1 may provide scientific basis for the development of CA16 vaccine.
【作者单位】：浙江大学医学院附属第一医院检验科;浙江省临床体外诊断技术研究重点实验室;中国人民解放军第一一七医院检验科;
【基金】：国家科技重大专项“传染病监测技术平台”项目(2012ZX10004-210)
【分类号】：R440
【正文快照】： ~A24)和B组病毒的6个血清型(B1~B6),其中柯萨奇病毒A16(Coxsaekievirus A16,CA16)是引起儿童手足口病(hand,foot and mouth disease,HFMD)的主要病原体之一[1]。CA16感染除了引起轻症感染外,还可导致重症或死亡病例发生[2-3]。近年来浙江省HFMD发病率一直位居法定传染病前两位

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