逆转录PCR在泌尿生殖道沙眼衣原体药敏试验中的应用

发布时间：2017-12-31 16:15

本文关键词：逆转录PCR在泌尿生殖道沙眼衣原体药敏试验中的应用　出处：《中国皮肤性病学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的将逆转录聚合酶链式反应(RT-PCR)应用于沙眼衣原体药物敏感性实验,对沙眼衣原体的药敏实验进行优化。方法将经Mc Coy细胞培养法检测出的40例沙眼衣原体临床株,传代培养至感染率达90%以上,进行多西环素的药敏实验。采用传统碘染方法和RT-PCR法来测定多西环素药物的MIC值。采用t检验比较两种方法的区别。结果采用RT-PCR测定的MIC值要高于传统碘染方法(P0.05)。结论用RT-PCR代替传统碘染方法能够提高沙眼衣原体体外药物敏感实验的敏感性,有助于临床医生在临床治疗失败病例中更好地寻找耐药菌株。
[Abstract]:Objective to apply reverse transcriptase polymerase chain reaction RT-PCR to the drug sensitivity test of Chlamydia trachomatis (Chlamydia trachomatis). Methods 40 clinical strains of chlamydia trachomatis detected by MC Coy cell culture method were subcultured to the infection rate of more than 90%. The drug sensitivity test of doxycycline was carried out. The MIC value of doxycycline was determined by traditional iodine staining method and RT-PCR method. T test was used to compare the difference between the two methods. RT-PCR was used to determine the results. MIC is higher than that of traditional iodine staining. Conclusion using RT-PCR instead of traditional iodine staining method can improve the sensitivity of chlamydia trachomatis in vitro. It is helpful for clinicians to better search for resistant strains in cases of failure of clinical treatment.
【作者单位】：赣南医学院第一附属医院皮肤科;天津医科大学总医院;
【基金】：江西省卫生厅科技计划(20131782)
【分类号】：R440;R759
【正文快照】： 目前,沙眼衣原体(Chlamydia trachomatis,Ct)和淋球菌感染是世界范围内最常见的细菌性性传播疾病。热休克蛋白60(HSP60)在细胞的生命活动中具有重要的功能。当细胞处于应激条件时热休克蛋白表达量明显提高。衣原体在不利生长的条件下,为逃逸宿主的免疫防御产生一种可逆状态,称

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