硫化氢对高糖腹膜透析液诱导大鼠腹膜结构和功能损伤的影响

发布时间：2018-01-01 23:21

本文关键词：硫化氢对高糖腹膜透析液诱导大鼠腹膜结构和功能损伤的影响　出处：《肾脏病与透析肾移植杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的:观察硫化氢(H_2S)对高糖腹膜透析液诱导大鼠腹膜结构和功能损伤的影响。方法:SD大鼠随机分为四组(对照组、单独H_2S组、模型组及治疗组)。第28天行腹膜平衡试验;取壁层腹膜组织观察腹膜结构变化,检测α平滑肌肌动蛋白(α-SMA)、胶原蛋白Ⅲ(Col-Ⅲ)和转化生长因子β1(TGF-β1)表达情况。检测大鼠腹膜间皮细胞单核细胞趋化蛋白1(MCP-1)、白细胞介素6(IL-6)、细胞间黏附分子1(ICAM-1)、α-SMA、E-钙黏素(E-cadherin)和TGF-β1 mRNA表达情况。结果:与对照组相比,模型组腹膜增厚伴血管增生、炎症细胞浸润增加,且间皮下α-SMA、Col-Ⅲ及TGF-β1表达显著增加(P0.05);与模型组相比,治疗组腹膜形态明显改善,α-SMA、Col-Ⅲ及TGF-β1表达显著减少(P0.05)。与对照组相比,模型组超滤量显著减少,腹膜通透性显著升高,治疗组大鼠腹膜转运功能显著改善(P0.05)。2.5%葡萄糖透析液刺激腹膜间皮细胞12h使肌成纤维细胞标志蛋白α-SMA表达显著增加、上皮细胞标志蛋白E-cadherin表达显著减少,同时MCP-1、IL-6、ICAM-1和TGF-β1 mRNA表达显著增加(P0.01);透析液中加入100μmol/L、300μmol/L NaHS可显著抑制上述炎症因子及纤维化因子表达(P0.01)。结论:在腹膜透析液中加入外源性H_2S可显著改善腹膜结构及功能损伤,并可部分逆转腹膜间皮细胞转分化,进而减少炎症因子和纤维化因子合成。
[Abstract]:Objective: To observe the effect of hydrogen sulfide (H_2S) induced by peritoneal structure and function injury in rats with high glucose and Liquor Dialysisintraperitoneus. Methods: SD rats were randomly divided into four groups (control group, H_2S alone group, model group and treatment group). Twenty-eighth days after peritoneal equilibration test; peritoneum observing peritoneal structural change detection alpha smooth muscle actin (alpha -SMA), collagen III (Col- III) and transforming growth factor beta 1 (TGF- beta 1). The detection of expression of rat peritoneal mesothelial cells of monocyte chemotactic protein 1 (MCP-1), interleukin 6 (IL-6), intercellular adhesion molecule 1 (ICAM-1), alpha -SMA, E- cadherin (E-cadherin) and TGF- beta 1 expression of mRNA. Results: compared with control group, model group, peritoneal thickening with vascular proliferation, increased infiltration of inflammatory cells, and submesothelial alpha -SMA significantly increased Col- III and TGF- beta 1 expression (P0.05); compared with the model group, the treatment group of peritoneum morphology improved, alpha -SM A, Col- III and TGF- beta 1 expression was significantly reduced (P0.05). Compared with the control group, model group, ultrafiltration volume was significantly reduced, peritoneal permeability increased significantly in treatment of peritoneal transport function in rats was significantly improved (P0.05).2.5% glucose dialysate stimulated peritoneal mesothelial cells 12h muscle fibroblast cells significantly increased expression of protein markers alpha -SMA, epithelial cell marker protein expression of E-cadherin decreased significantly, while MCP-1, IL-6, ICAM-1 and TGF- significantly increased the expression of mRNA beta 1 (P0.01); adding 100 mol/L dialysate, 300 mol/L NaHS can significantly inhibit the expression of inflammatory factor and fibrosis factor (P0.01). Conclusion: the addition of exogenous H_2S in Liquor Dialysisintraperitoneus can significantly improve the structure and function of peritoneal injury, and can partially reverse the transdifferentiation of peritoneal mesothelial cell, thereby reducing the inflammation and fibrosis factor synthesis.

【作者单位】：苏州大学附属第二医院肾内科;
【基金】：国家自然科学基金(青年科学基金)(81400762) 苏州市科技计划项目基金(应用基础研究)(SYS201469)
【分类号】：R692.5
【正文快照】： 腹膜透析(PD)是终末期肾病患者主要替代治疗方法之一。生物不相容的腹膜透析液(PDF)和反复发生的腹膜炎可引起腹膜纤维化,致使PD患者退出PD治疗。因此,寻找有效的抗腹膜纤维化药物是PD基础研究的热点之一。研究表明多种器官纤维化过程中伴有内源性硫化氢(H2S)缺乏[1-3]。我们

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