健康人外周血白念珠菌果糖二磷酸醛缩酶的抗原特异性T细胞检测

发布时间：2018-01-04 21:36

本文关键词：健康人外周血白念珠菌果糖二磷酸醛缩酶的抗原特异性T细胞检测　出处：《医学研究生学报》2017年06期 　论文类型：期刊论文

【摘要】：目的侵袭性念珠菌病临床致死率较高,文中旨在研究白念珠菌免疫优势抗原1,6-果糖二磷酸醛缩酶(Fba)的细胞免疫应答类型,以评估其在侵袭性念珠菌病中是否具有保护性细胞免疫功能。方法建立检测健康人外周血单个核细胞(PBMC)中白念珠菌Fba抗原特异性T淋巴细胞分泌干扰素γ(IFN-γ)、白细胞介素-4(IL-4)与白细胞介素-17A(IL-17A)的酶联免疫斑点法(ELISPOT)。用白念珠菌重组抗原Fba为刺激物,检测26名健康人外周血PBMC中分泌IFN-γ、IL-4和IL-17A的阳性斑点形成细胞(SFC)频数。结果 Fba刺激后,26名健康人分泌IFN-γ、IL-4和IL-17A的频数分别为23.00(9.75,42.50)个、0(0,0.25)个和1.5(0.75,8.25)个,三者间两两比较差异均有统计学意义(P0.01)。IFN-γ、IL-4和IL-17A的应答率分别为100%(26/26)、15.38%(4/26)和76.92%(20/26),IFN-γ和IL-17A的应答率显著高于IL-4应答率(P0.01)。其中Fba刺激后产生强IFN-γ应答(SFC≥20)者占57.69%(15/26),而产生IL-17A强应答者1人,无人产生强IL-4应答。健康受试者PBMC应答同时产生Th1和Th17型应答者占65.38%(17/26);其中19.23%(5/26)的受试对象仅产生Th1型细胞免疫应答,无受试对象单独产生Th2型细胞免疫应答。结论白念珠菌特异性抗原Fba诱导的T细胞免疫以Th1型和Th17型为主,提示该抗原将产生保护性细胞免疫应答,具有成为理想疫苗的潜能。
[Abstract]:Objective to study the type of cellular immune response of Candida albicans immune dominant antigen 1 ~ (6) fructose diphosphate aldolase (Fba). To evaluate the protective cellular immune function in invasive candidiasis. Methods PBMCs of peripheral blood mononuclear cells from healthy individuals were established. Medium Candida albicans Fba antigen-specific T lymphocytes secreted interferon 纬 (IFN- 纬). The enzyme linked immunoblot assay (Elisa) of interleukin-4 (IL-4) and interleukin-17A (IL-17A). The recombinant antigen Fba of Candida albicans was used as a stimulant. The frequency of IFN- 纬 IL-4 and IL-17A positive spot forming cells (SFCs) secreted from peripheral blood PBMC of 26 healthy people was determined. Results after Fba stimulation, the frequency of IFN- 纬 IL-4 and IL-17A positive spot forming cells was detected. The frequency of IFN- 纬 IL-4 and IL-17A secreted from 26 healthy people were 23.00 ~ 9.75 ~ 42.50) 0.25) and 1.5 ~ 0.75 respectively. The response rates of IL-4 and IL-17A were 100 / 26 / 26 respectively. 15.38 (4 / 26) and 76.92 (20 / 26). The response rates of IFN- 纬 and IL-17A were significantly higher than those of IL-4 (P 0.01). 15 / 26. One person produced strong IL-17A response, none produced strong IL-4 response. In healthy subjects, both Th1 and Th17 type of PBMC response were found to be 65.38% or 17 / 26%. Of these, 19.23% of the subjects had only Th1 type cellular immune responses. Conclusion the T cell immunity induced by Candida albicans specific antigen Fba was mainly Th1 type and Th17 type. The results suggest that the antigen will produce protective cellular immune response and have the potential to be an ideal vaccine.
【作者单位】：南京军区南京总医院全军临床检验研究所;
【基金】：国家自然科学基金(81302536) 南京军区医药卫生科研基金(15MS125)
【分类号】：R446.6;R519
【正文快照】： 0引言白念珠菌是人体的正常菌群之一,可定植于人体皮肤表面、消化道、呼吸道和女性阴道黏膜。在机体免疫功能减退或正常生理屏障被破坏后,可引起危及生命的侵袭性念珠菌病(invasive candiasis,IC)。近年来,随着艾滋病的流行、临床恶性肿瘤、器官移植患者的增多,以及侵入性临床

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