iPS细胞来源的嵌合体胸腺移植促进异基因骨髓移植小鼠的T细胞重建和抑制移植物抗宿主病

发布时间：2018-01-07 22:27

本文关键词：iPS细胞来源的嵌合体胸腺移植促进异基因骨髓移植小鼠的T细胞重建和抑制移植物抗宿主病　出处：《实用医学杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的:探讨诱导性多能干细胞(i PS细胞)来源的嵌合体胸腺移植(TT)对异基因骨髓移植小鼠的T细胞重建和移植物抗宿主病(GVHD)的影响。方法:将i PS细胞来源的嵌合体胸腺,植入异基因骨髓内骨髓移植(IBM-BMT)受体小鼠的肾被膜下,4周后分析比较IBM-BMT组、IBM-BMT+TT组、IBM-BMT+淋巴细胞输注(DLI)组的T细胞亚群、GVHD临床评分和病理学检查。结果:IBM-BMT组、IBM-BMT+TT组、IBM-BMT+DLI组外周血CD8+T细胞比例分别为(5.52±0.83)%、(11.10±1.49)%、(8.49±0.82)%,组间差异有统计学意义(P0.05)。IBM-BMT+TT组的CD4+T细胞比例为(9.60±0.69)%,高于IBM-BMT组(6.42±1.40)%和IBM-BMT+DLI组(8.07±0.65)%(P0.05)。IBM-BMT组、IBM-BMT+TT的GVHD评分低于IBM-BMT+DLI组,病理表现更轻。结论:i PS细胞来源的嵌合体胸腺移植,能有效加快移植后T细胞重建,并抑制GVHD反应。
[Abstract]:Objective: To investigate the effect of induced pluripotent stem cells (I PS cells) chimeric thymus transplantation derived T cells (TT) reconstruction of mouse allogeneic bone marrow transplantation and graft-versus-host disease (GVHD). Methods: the effects of chimeric thymus I cell PS, implantation of allogeneic bone marrow in bone marrow transplantation (IBM-BMT) mice under the kidney capsule, analysis and comparison of IBM-BMT group, IBM-BMT+TT group after 4 weeks, IBM-BMT+ lymphocyte infusion (DLI) group, T cell subsets, GVHD clinical score and pathological examination. Results: in IBM-BMT group, IBM-BMT+TT group, the percentage of CD8+T cells in peripheral blood IBM-BMT+DLI group respectively (5.52. 0.83)% and (11.10 + 1.49)% and (8.49 + 0.82)%, there was significant difference between the groups (P0.05) the proportion of CD4+T cells in the.IBM-BMT+TT group (9.60 + 0.69)%, higher than that of group IBM-BMT (6.42 + 1.40)% and IBM-BMT+DLI group (8.07 + 0.65)% (P0.05).IBM-BMT group IBM-BMT+TT, the GVHD score was lower than IBM-BMT+DLI Conclusion: I PS cell derived chimeric thymus transplantation can effectively accelerate the reconstruction of T cells after transplantation and inhibit the reaction of GVHD.

【作者单位】：南方医科大学南方医院小儿科;广东医科大学;
【基金】：国家自然科学基金(编号:81270632,81302544) 广东省扬帆计划引进紧缺拔尖人才项目(编号:201433004) 广东医学院人才引进人员科研启动项目(编号:B2013015) 留学人员科技活动项目择优资助(编号:2QT14002)
【分类号】：R457.7
【正文快照】： 加快异基因骨髓移植(allogeneic bone marrow transplantation,allo-BMT)后T细胞重建能够降低感染和肿瘤复发的几率,对受体具有重要意义,临床常用的方法是供体淋巴细胞输注(donor lympho-cyte infusion,DLI)[1-4],DLI能够在allo-BMT早期给受体提供免疫保护、应对感染威胁、具

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