抗-C、e同种抗体合并抗-e类同种自身抗体的鉴定及交叉配血

发布时间：2018-01-08 20:31

本文关键词：抗-C、e同种抗体合并抗-e类同种自身抗体的鉴定及交叉配血　出处：《中国输血杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的通过对1例同时产生了类同种自身抗体和同种抗体患者的抗体分析及鉴定,了解Rh血型系统中同种抗体、简单特异性自身抗体和类同种自身抗体的血清学特征,从血清学上对三者进行区分,以便采取更适合的红细胞成分输血策略。方法采用筛选细胞和谱细胞、以不同方法对患者血清进行抗体筛查及鉴定。采用患者Rh抗体类似特异性对应抗原阴性的O型RhccDEE的献血者细胞、添加低离子液(LISS)的吸收方法,对患者吸收后的血清、红细胞放散液进行抗体鉴定。对以往相关文献进行回顾。结果患者Rh分型ccDEE,直抗阳性。血清抗体鉴定为IgM类的抗-C、抗-e和IgG类的抗-e(不排除抗-Ce可能性)。吸收后血清抗体鉴定为IgM类的抗-C、抗-e(不排除抗-Ce可能性)和IgG类的抗-e。放散液抗体鉴定为IgG类的抗-e。结论对患者红细胞Rh表型、直接抗球蛋白试验,以及分别对患者血清、吸收后血清及放散液抗体特异性进行综合分析,可区分出同种抗体或类同种抗体。而采用添加低离子液(LISS)的方法可更好的吸收低亲和力抗体。
[Abstract]:At the same time through analysis and identification of antibodies in patients with the same antibodies and isotype antibody in 1 cases, understand the same Rh system antibody, serological features simple specific autoantibodies and the same autoantibodies, distinguished from serological to three, in order to take red blood cells transfusion strategy is more suitable for the methods screening cells and spectrum of cells, screening and identification of serum antibody by different methods. O RhccDEE using Rh antibody in patients with similar specificity of the corresponding antigen negative blood donor cells, add low ionic liquid (LISS) absorption method of serum antibodies were identified after absorption, emission of red blood cells liquid. The review of the previous relevant literature. Results in patients with Rh type ccDEE, direct anti serum antibody positive. Identification of anti -C IgM, anti -e and anti IgG -e (do not rule out the possibility of anti -Ce) after absorption. Identification of serum antibody for anti -C IgM, anti -e (do not rule out the possibility of anti -Ce and anti -e.) elution antibody identification of class IgG anti -e. conclusion IgG on red blood cell in patients with Rh phenotype, direct antiglobulin test, and comprehensive analysis on serum, serum and elution specific antibody for absorption, can be divided into the same class. And the antibody or isotype antibody by adding low ionic liquid (LISS) method can better absorb low affinity antibodies.

【作者单位】：湖州市中心血站;
【分类号】：R457.1
【正文快照】： #共同第一作者Rh是血型系统中最复杂的,包括从编号Rh1-61共54个抗原,以及7个已弃用抗原[1]。Rh抗原也是温自身抗体最常见的靶抗原,这些特异性偶尔出现,但大多数情况下,要查明AIHA患者红细胞和血清中的这种特异性,使用Rh表型细胞进行吸收放散试验是必需的,而有些看似简单Rh特异

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