新型MRI对比剂钆喷酸掺杂的介孔二氧化硅纳米粒的制备、表征及MR成像

发布时间：2018-01-12 13:40

本文关键词：新型MRI对比剂钆喷酸掺杂的介孔二氧化硅纳米粒的制备、表征及MR成像　出处：《重庆医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的研制一种新型的具有分子靶向显像潜能的高掺钆量的介孔二氧化硅纳米粒子用于磁共振成像。方法采用反向微乳液法将钆喷酸(Gadopentetic acid,GA)加入到微乳液体系中,从而将GA掺杂到介孔二氧化硅(mesoporous silica nanoparticles,MSN)的孔道中,制备出掺杂GA的二氧化硅纳米材料(Gd-MSN);同时制备不含钆的MSN为对照。用马尔文粒度仪、透射电镜(Transmission electron microscope,TEM)观察纳米粒的粒径及形貌特征;电感耦合等离子体原子发射光谱分析(Inductively Coupled Plasma-Atomic Emission Spectrometry,ICP-AES)检测纳米粒的含钆量和稳定性;CCK-8(Cell Counting Kit-8,CCK-8)法检测纳米粒的细胞毒性;最后用3.0T磁共振成像系统观察纳米粒的体内外磁共振成像效果。结果成功制备出钆喷酸掺杂的二氧化硅纳米材料Gd-MSN,TEM观察Gd-MSN形态呈圆球形,马尔文测得其平均粒径约105-128nm;ICP-AES测得掺钆量最高可达771.08μmo L/g,Gd-MSN在体外环境中具有良好的稳定性,且在25,50,100μg/m L的浓度下没有明显的细胞毒性;体内外磁共振结果显示Gd-MSN能显著缩短T1和T2的弛豫时间,表现为短T1短T2的信号特点。结论成功制备了单分散、易修饰、高载药量的新型掺钆纳米材料,有望成为一种新型的多功能靶向磁共振纳米对比剂。
[Abstract]:Objective to develop a new type of molecular targeted high amount of potential imaging gadolinium doped mesoporous silica nanoparticles for magnetic resonance imaging. Methods using reverse microemulsion method gadopentetic acid (Gadopentetic acid, GA) added to the micro emulsion system, thus to the GA doped mesoporous silica (mesoporous silica, nanoparticles, MSN) in the channels of the prepared silica nanomaterials doped with GA (Gd-MSN); at the same time preparing gadolinium containing MSN as control. Using the Malvin particle analyzer, transmission electron microscopy (Transmission electron, microscope, TEM) to observe the nanoparticles size and morphology; inductively coupled plasma atomic emission spectrometry (Inductively Coupled Plasma-Atomic Emission Spectrometry, ICP-AES) gadolinium content and stability were detected; CCK-8 (Cell Counting Kit-8 CCK-8) method was used to detect the cytotoxicity of nanoparticles; finally using 3.0T magnetic resonance MRI system observation in vivo magnetic resonance imaging effect of nanoparticles. The prepared silica nanomaterials Gd-MSN gadopentetic acid doped TEM, Gd-MSN spherical morphology observation, Malvin measured the average particle size of about 105-128nm; ICP-AES doped gadolinium content measured up to 771.08 Mo L/g, Gd-MSN has a good stability in vitro environment, and there is no obvious cytotoxicity in the concentration of 25,50100 g/m L; in vitro and in vivo MRI results show that Gd-MSN can significantly reduce the T1 and T2 relaxation time for signal characteristics of short T1 and short T2. Conclusion the successful preparation of monodisperse, easy modification, new gadolinium doped nano material high loading, is expected to become a new target to multi functional magnetic resonance nano contrast agent.

【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R445.2

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