沙门菌tcpS基因的血清型分布及其在特定血清型鉴定中的应用

发布时间：2018-01-13 00:03

本文关键词：沙门菌tcpS基因的血清型分布及其在特定血清型鉴定中的应用　出处：《中国人兽共患病学报》2017年09期 　论文类型：期刊论文

更多相关文章： 沙门菌 tcpS基因 PCR 血清型

【摘要】：目的建立基于tcpS基因的PCR鉴定方法,同时筛选肠炎沙门菌、鸡白痢/伤寒沙门菌和都柏林沙门菌,为流行病学调查提供方便快速的实验室技术支持,并为PCR鉴定沙门菌方法的发展提供新的基因候选。方法利用生物信息学的方法分析沙门菌tcpS基因的血清型分布特点,选择27种不同血清型沙门菌以及10株非沙门菌菌株,对所建立PCR体系的特异性和灵敏性进行检测,并将该方法应用于江苏省分离的48株猪场分离株、22株鸡场分离株和11株牛场分离株的检测。结果生物信息学分析表明tcpS基因仅存在于肠炎沙门菌、鸡白痢/伤寒沙门菌和都柏林沙门菌中,该PCR方法具有高度特异性和灵敏性,可准确快速地从临床样本中筛选这3种血清型沙门菌,且对实际样本检测符合率达100%。结论基于tcpS的PCR检测方法可准确筛选肠炎沙门菌、鸡白痢/伤寒沙门菌和都柏林沙门菌,能作为传统血清学分型的辅助方法,为沙门菌PCR鉴定方法的发展提供了优良的基因候选。
[Abstract]:Objective to establish PCR identification method based on tcpS gene and screen Salmonella enteritidis, Salmonella pullorum / Salmonella typhimurium and Salmonella Dublin at the same time, so as to provide convenient and rapid laboratory technical support for epidemiological investigation. New gene candidates were provided for the development of PCR method for the identification of Salmonella. Methods the distribution characteristics of serum type of Salmonella tcpS gene were analyzed by bioinformatics. The specificity and sensitivity of the established PCR system were determined by selecting 27 different serotypes of Salmonella and 10 non-Salmonella strains. The method was applied to 48 pig farm isolates isolated from Jiangsu Province. Results Bioinformatics analysis showed that tcpS gene only existed in Salmonella enteritidis, Salmonella pullorum / Salmonella typhimurium and Salmonella Dublin. The PCR method has high specificity and sensitivity and can be used to screen the three serotype Salmonella serotype from clinical samples accurately and quickly. Conclusion the PCR method based on tcpS can be used to screen Salmonella enteritidis, Salmonella pullorum / Salmonella typhimurium and Salmonella Dublin accurately. It can be used as the assistant method of traditional serological typing, and it provides excellent gene candidates for the development of PCR identification method of Salmonella.
【作者单位】：江苏省人兽共患病学重点实验室/江苏省动物重要疫病与人兽共患病防控协同创新中心/农业与农产品安全国际合作联合实验室/农业部农产品质量安全生物性危害因子(动物源)控制重点实验室;
【基金】：国家重点研发计划课题(2016YFD0501607) 公益性行业(农业)科研专项(201403054) 国家自然科学基金(31320103907,31230070) 江苏省重点研发项目(BE2015343) 江苏省第9批“六大人才高峰”高层次人才项目(NY-028) 扬州大学科技创新团队江苏省高校优势学科建设工程项目联合资助~~
【分类号】：R440;R516.3
【正文快照】： Funded by the National Key Research and Development Program Special Project(No.2016YFD0501607),the Special Fund forAgroscientific Research in the Public Interest(No.201403054),the National Natural Science Foundation of China(Nos.31320103907and 31230070),

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