支气管哮喘患者外周血单个核细胞TIPE2、TF的表达

发布时间：2018-01-17 15:03

本文关键词：支气管哮喘患者外周血单个核细胞TIPE2、TF的表达　出处：《郑州大学学报(医学版)》2017年06期 　论文类型：期刊论文

【摘要】：目的:检测支气管哮喘患者外周血单个核细胞(PBMCs)肿瘤坏死因子-α诱导蛋白-8样因子-2(TIPE2)、组织因子(TF)表达水平及二者的相关性,并探讨二者与哮喘控制水平的相关性。方法:108例哮喘患者根据哮喘控制水平分为未控制组53例,部分控制组55例,另选择同时期健康体检者51人(健康对照组)。Western blot法检测3组患者PBMCs中TIPE2、TF的表达水平,Spearman秩相关分析二者的相关性,受试者工作曲线评估TIPE2和TF对支气管哮喘的诊断价值。结果:与部分控制组和健康对照组相比,未控制组PBMCs中TIPE2表达水平降低(P0.01);与健康对照组相比,部分控制组PBMCs中TIPE2表达水平降低(P0.01)。与健康对照组相比,部分控制组和未控制组PBMCs中TF表达水平升高(P0.01),而二组间差异无统计学意义(P0.05)。哮喘患者PB-MCs中TIPE2与TF的表达水平呈负相关
[Abstract]:Objective: to detect the tumor necrosis factor- 伪 inducible protein-8 (TIPE2) in peripheral blood mononuclear cells (PBMCs) from patients with bronchial asthma. Methods one hundred and eight patients with asthma were divided into uncontrolled group (53 cases) according to asthma control level. 55 cases of partial control group and 51 cases of health check at the same period were selected. The expression of TIPE2TF in PBMCs of three groups was detected by Western blot method. The diagnostic value of TIPE2 and TF in bronchial asthma was evaluated by Spearman rank correlation analysis. Results: compared with partial control group and healthy control group. The level of TIPE2 expression in PBMCs was decreased in uncontrolled group (P 0.01). Compared with the healthy control group, the level of TIPE2 expression in PBMCs in some control groups was lower than that in the healthy control group (P 0.01). The level of TF expression in PBMCs was increased in partial control group and non-control group (P 0.01). However, there was no significant difference between the two groups. There was a negative correlation between the expression of TIPE2 and TF in PB-MCs of asthmatic patients.
【作者单位】：郑州大学第一附属医院检验科;河南省检验医学重点实验室;郑州大学医学检验系;
【基金】：国家自然科学基金资助项目81501715 河南省科技攻关项目162102310142,152102410067
【分类号】：R446.1;R562.25
【正文快照】： 支气管哮喘(简称哮喘)是由多种细胞(T淋巴细胞、肥大细胞、嗜碱性粒细胞、嗜酸性粒细胞、巨噬细胞、中性粒细胞和上皮细胞)及细胞组分参与的慢性气道炎症性疾病。随着全球工业化和气候变化,哮喘发病率呈逐年上升的趋势[1]。哮喘气道炎症导致气流受限、气道高反应性和症状反复

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