基于棉线的可视化快速免疫层析分析技术的研究

发布时间：2018-01-18 08:33

本文关键词：基于棉线的可视化快速免疫层析分析技术的研究　出处：《西北大学》2017年硕士论文　论文类型：学位论文

【摘要】：本论文构建了一种以棉线为载体的免疫层析分析技术,利用不同的纳米材料,对肺癌标志物(鳞状癌细胞抗原、人铁蛋白)实现了快速、灵敏和可视化检测。具体工作如下:(1)基于碳纳米管探针的棉线免疫层析分析技术在本文中,成功地设计了一种基于碳纳米管(CNT)探针的棉线免疫分析技术,实现了可视化、快速检测肺癌标志物-人铁蛋白抗原。该种装置在30分钟内可以检测到50 ng/mL人铁蛋白抗原,与先前的基于碳纳米管探针的快速检测文献结果比较,灵敏度提高了大约500倍。(2)设计了一种基于双纳米金信号探针的棉线免疫传感器,用于电化学测定肺癌标志物-鳞状细胞癌抗原(Squamous Cell Carcinoma Antigen,SCCA)通过使用两种金纳米粒子标记物来改善SCCA检测灵敏度,与传统的采用一种金纳米粒子为信号探针的棉线免疫层析分析技术比较,大大提高了分析的灵敏度。整个分析过程避免了在大多数传统的蛋白质分析中的繁琐重复的温育和洗涤步骤,缩短了分析时间和降低了成本。在最佳的实验条件下,线性范围为5-500 ng/mL。(3)基于金纳米棒探针的棉线免疫层析分析技术本文设计了一种基于棉线免疫层析分析装置结合金纳米棒(GNRs)信号探针可视化、快速、灵敏的检测人铁蛋白抗原(Human ferritin)的方法。人铁蛋白是肺癌蛋白质生物标志物,对于肺癌早期检测具有重要的意义。结果表明,与传统的金纳米粒子探针相比,由于金纳米棒独特的光学性质,使得我们设计的棉线免疫层析分析装置的灵敏度提高了近1000倍。在最佳条件下,棉线免疫层析分析装置可以检测到5 ng/mL人铁蛋白抗原,满足临床诊断的要求。
[Abstract]:In this paper, an immunochromatographic technique based on cotton thread was constructed, and the marker of lung cancer (squamous carcinoma cell antigen, human ferritin) was quickly achieved by using different nano-materials. Sensitive and visual detection. The specific work is as follows: 1) the cotton thread immunochromatographic technique based on carbon nanotube probe is presented in this paper. A cotton thread immunoassay based on CNT probe was successfully designed and visualized. Rapid detection of human ferritin antigen, a marker of lung cancer. The device could detect 50 ng/mL human ferritin antigen within 30 minutes. Compared with the previous rapid detection results based on carbon nanotube probes, the sensitivity is increased by about 500 times.) A cotton thread immunosensor based on double nanoscale gold signal probe is designed. It was used for electrochemical determination of squamous Cell Carcinoma Antigen, a marker of lung cancer. The sensitivity of SCCA detection was improved by using two kinds of gold nanoparticles, compared with the traditional cotton thread immunochromatographic technique using a gold nanoparticles as signal probe. The sensitivity of the analysis is greatly improved. The whole analysis process avoids the tedious and repetitive steps of temperature and washing in most traditional protein analysis. The analysis time is shortened and the cost is reduced. Linear range 5-500 ng / m 路L ~ (3) A cotton thread immunochromatographic technique based on gold nanorod probe was designed in this paper based on a cotton thread immunochromatographic apparatus combined with gold nanorods GNRs). Signal probe visualization. A rapid and sensitive method for the detection of human ferritin antigen (human ferritin). Human ferritin is a biomarker of lung cancer protein. The results show that compared with the traditional gold nanoparticles probe, gold nanorods have unique optical properties. The sensitivity of the cotton thread immunochromatographic analysis device was improved by nearly 1, 000 times. Under the optimal conditions, 5 ng/mL human ferritin antigen could be detected by the cotton thread immunochromatographic analysis device. To meet the requirements of clinical diagnosis.
【学位授予单位】：西北大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R446.6;O652

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