顶空-固相微萃取-气相色谱法测定尿中丁酮

发布时间：2018-01-19 08:03

  本文关键词： 顶空固相微萃取 气相色谱 丁酮 尿　出处：《现代预防医学》2017年04期 　论文类型：期刊论文

【摘要】：目的建立顶空-固相微萃取-气相色谱法测定尿中丁酮的方法。方法利用顶空技术,将适量盐析剂和待测丁酮溶液置于20 ml顶空瓶中,混合均匀后置于一定温度的水浴锅中,经DVB/CAR/PDMS固相微萃取萃取头吸附顶空气中的丁酮,待平衡后将纤维头取出、插入气相色谱气化室,热解吸涂层上吸附的丁酮,经毛细管色谱柱分离,FID检测器检测。结果 DVB/CAR/PDMS萃取头、20 min萃取时间、30℃平衡温度、2.0g Na Cl盐析条件下,丁酮的测定范围为0.5~5.0μg/ml,相关系数r=0.9999,方法检出限为0.005μg/ml,精密度0.72%~4.63%,方法回收率94.7%~101.2%。结论该方法各指标满足我国生物材料中化学物质测定方法的要求,适用于职业接触丁酮劳动者尿中丁酮的检测。
[Abstract]:Objective to establish a method for the determination of butanone in urine by headspace solid phase microextraction gas chromatography. Methods proper amount of salting-out agent and butanone solution were placed in 20ml headspace bottle by headspace technique. The mixture was evenly mixed and placed in a water bath at a certain temperature. The butanone in the top air was adsorbed by the DVB/CAR/PDMS solid-phase microextraction head. The fiber head was removed after equilibrium and inserted into the gas chromatographic gasification chamber. Butanone adsorbed on the thermal desorption coating was determined by capillary column separation and FID detector. Results the extraction time of DVB/CAR/PDMS extractor was 20 min and the equilibrium temperature was 30 鈩，

本文编号：1443315