2016年北京地区急性腹泻患儿感染人博卡病毒流行特征及分子进化研究

发布时间：2018-01-20 10:59

本文关键词： 急性腹泻人博卡病毒进化分析　出处：《中国病原生物学杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的了解2016年北京地区急性腹泻患儿感染人博卡病毒(HBoV)流行及遗传进化特征。方法采集北京地区哨点医院2016年60月龄急性腹泻患儿粪便标本,用巢式PCR检测HBoV,通过测序和Blast比对确定病毒型别,并进行遗传进化分析;采用实时荧光PCR对A组轮状病毒、诺如病毒、肠道腺病毒、星状病毒进行检测,分析混合感染情况。结果 354份粪便标本中,HBoV阳性26份,阳性率为7.34%,其感染无明显季节分布,2岁患儿占84.62%。HBoV与其他腹泻病毒混合感染率为38.46%,以混合轮状病毒感染为主。进化分析表明,HBoV1、2、3型感染分别为15、10、1例,未检出HBoV4型。HBoV2序列均独立于HBoV2A和HBoV2B亚型分支,位于一个新的HBoV2亚型分支上。结论 2016年北京地区腹泻患儿感染HBoV包括3种型别(1-3型),2岁以内患儿是高发人群。HBoV2在进化树上位于一个新的亚型分支。
[Abstract]:Objective to investigate the infection of human Boca virus HBoV in children with acute diarrhea in Beijing in 2016. Methods the feces of 60 months old children with acute diarrhea in 2016 were collected from the sentinel hospital of Beijing area. Nested PCR was used to detect HBoV.The virus type was determined by sequencing and Blast comparison, and genetic evolution analysis was carried out. Group A rotavirus, norovirus, enteroadenovirus and stellate virus were detected by real-time fluorescence PCR. Results 26 of 354 fecal specimens were positive for HBoV. The positive rate of HBoV was 7.34. The infection rate of children aged 2 years with no obvious seasonal distribution was 84.62%. The mixed infection rate of HBoV and other diarrhea virus was 38.46%. Phylogenetic analysis showed that 1 51010 cases were infected with HBoV1 / 2G type 3, respectively, and 1 case was infected with mixed rotavirus infection. No HBoV4 type. HBoV2 sequence was found to be independent of HBoV2A and HBoV2B subtypes. It is located on a new branch of HBoV2 subtype. Conclusion in 2016, children with diarrhea in Beijing area were infected with HBoV including three types of serotype 1-3). Children under 2 years of age are a high risk population. HBoV 2 is located in a new subtype branch of the phylogenetic tree.
【作者单位】：北京市西城区疾病预防控制中心;
【基金】：北京市西城区卫计委青年科技人才培养项目(No.XWKX2016-17)
【分类号】：R440;R725.1
【正文快照】： ***急性感染性腹泻是儿童多发传染病,也是我国常见的丙类传染病之一,其发病率和病死率高[1-2]。病毒是婴幼儿急性腹泻的主要病原,常见的有诺如病毒(novovirus,NV)、轮状病毒(rotavirus,RV)、肠道腺病毒(adenovirus,AdV)和星状病毒(astrovirus,AstV)等。但尚有较高比例婴幼儿急

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