外阴阴道假丝酵母菌病来源的热带假丝酵母菌多位点序列分型

发布时间：2018-01-20 14:53

本文关键词： 外阴阴道假丝酵母菌病热带假丝酵母菌多位点序列分型药物敏感性耐药基因　出处：《现代妇产科进展》2017年08期 　论文类型：期刊论文

【摘要】：目的:应用多位点序列分型(MLST)分析38株热带假丝酵母菌的亲缘关系,体外比较热带假丝酵母菌对常见抗真菌药物的敏感性,检测耐药基因的表达水平。方法:通过对6对等位基因进行测序,用e BURST分组和非加权配对算术平均法(UPGMA)聚类分析菌株的进化和变异。微量肉汤稀释法进行体外抗真菌药物敏感试验,实时荧光定量聚合酶链反应(q-RT-PCR)检测耐药基因CDR1、CDR2、ERG1、ERG3、ERG6、ERG9和ERG11表达。结果:38株菌株中获得30个二倍体序列(DST),其中14个新DST,11个新等位基因,27个DST为单体型。e BURST分组,主要分成2组,大多数DST属于单体型。聚类分析总共分18个进化枝。阿尼芬净、米卡芬净、卡泊芬净、氟康唑、伏立康唑、伊曲康唑、两性霉素B、氟胞嘧啶、咪康唑、布康唑、克霉唑、特康唑、特比萘芬和制霉菌素14种抗真菌药物的MIC90分别为0.030μg/ml、0.015μg/ml、0.500μg/ml、1.000μg/ml、0.125μg/ml、0.250μg/ml、1.000μg/ml、0.250μg/ml、2.000μg/ml、2.000μg/ml、0.250μg/ml、0.250μg/ml、256.000μg/ml和8.000μg/ml。绝大多数耐药基因表达量较高的菌株可能在进化枝11和14。结论:热带假丝酵母菌MLST变异较大,菌株之间遗传距离较远。热带假丝酵母菌对特比萘芬普遍耐药,对目前其他常用的抗真菌药物多为敏感。
[Abstract]:Objective: to analyze the relationship of 38 strains of Candida tropicalis by multilocus sequence typing (MLST), and to compare the susceptibility of Candida tropicalis to common antifungal drugs in vitro. Methods: six pairs of alleles were sequenced. The evolution and variation of strains were analyzed by e BURST grouping and unweighted pairing arithmetic average method. In vitro antifungal susceptibility test was carried out by broth dilution method. Real-time fluorescence quantitative polymerase chain reaction (RT-PCR) was used to detect the resistance gene CDR1, CDR2ERG1, ERG3 and ERG6. ERG9 and ERG11 expression. Results 30 diploid sequences were obtained from 38 strains, of which 14 new DSTs and 11 new alleles were obtained. 27 DST were divided into haplotype. E BURST groups, and most of them were haplotypes. Cluster analysis consisted of 18 evolutionary branches. Fluconazole, Volconazole, itraconazole, amphotericin B, Fluocytosine, Miconazole, buconazole, clotrimazole, Teconazole. The MIC90 of terbinafine and nystatin were 0.030 渭 g / ml, 0.015 渭 g / ml and 0.500 渭 g / ml, respectively. 0.125 渭 g / ml 0.250 渭 g / ml 1.000 渭 g / ml 0.250 渭 g / ml 2.000 渭 g / ml 2.000 渭 g / ml. 0.250 渭 g / ml 0.250 渭 g / ml. 256.000 渭 g / ml and 8.000 渭 g / ml. Most strains with high expression of drug-resistant genes may be in Evolutionary branches 11 and 14. Conclusion:. The MLST variation of Candida tropicalis was significant. Candida tropicalis is generally resistant to terbinafine and is sensitive to other commonly used antifungal agents.
【作者单位】：安徽医科大学北京大学深圳医院临床学院;北京大学深圳医院;
【分类号】：R440;R711.3
【正文快照】： 外阴阴道假丝酵母菌病(vulvovaginal candidiasis,VVC)是由假丝酵母菌感染引起的最常见外阴阴道炎症,是育龄女性最常见的生殖道感染性疾病之一。75%育龄期妇女一生中至少感染过1次VVC,其发病率仅次于细菌性阴道病,50%妇女经历过VVC复发,5%~8%成年女性发生复发性外阴阴道假丝酵

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