标本溶血对生化检验结果影响的研究与探讨

发布时间：2018-01-21 09:38

本文关键词： 溶血生化检验标本溶血临床标本　出处：《中国当代医药》2017年09期 　论文类型：期刊论文

【摘要】：目的探讨标本溶血对生化检验结果的影响分析。方法选择2015年9~12月在我院门诊进行体检的100名正常志愿者的血液标本作为研究对象,采用日立7600-010型全自动生化分析仪检测血液标本溶血前后谷氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBil)、直接胆红素(DBil)、总蛋白(TP)、白蛋白(Alb)、肌酸激酶(CK)、K~+、Ca~(2+)、葡萄糖(Glu)、尿素氮(BUN)、尿酸(UA)等12项生化指标,观察溶血前后各项指标的水平差异。结果研究对象血液标本溶血前后AST、ALT、TBil、DBil、TP、ALB、CK、K~+、Glu、BUN水平比较,差异均有统计学的意义(P0.05);溶血前后血液中UA、Ca~(2+)水平比较,差异无统计学的意义(P0.05)。结论标本溶血现象对很多临床生化检验实验有很大的影响,为了尽可能减少或避免标本溶血的发生,要求临床工作人员采血时严格按照要求操作,对标本的采集、运送、分离以及检测过程严格操作,做好临床标本分析前的质量控制,使检验结果更准确可靠。
[Abstract]:Objective to investigate the effect of hemolysis on the results of biochemical examination. Methods the blood samples of 100 healthy volunteers who were examined in our outpatient clinic from 2015 to December were selected as the research objects. The glutamate aminotransferase (alt) and aspartate aminotransferase (AST) were detected by Hitachi 7600-010 automatic biochemical analyzer before and after hemolysis. Total bilirubin T bilirubin, direct bilirubin, total protein TTP, Alb, creatine kinase (CK) K, glucose Glu2). There were 12 biochemical indexes, such as urea nitrogen bun, uric acid UAA, and so on, to observe the difference of each index before and after hemolysis. Results the blood samples were collected before and after hemolysis. There were significant differences in the level of K ~ + Glun bun between the two groups (P < 0.05). Before and after hemolysis, there was no significant difference in the level of UAV Cam 2. Conclusion the hemolysis of the specimen has great influence on many clinical biochemical tests. In order to minimize or avoid the occurrence of specimen hemolysis, clinical staff are required to strictly follow the required operation, collection, transportation, separation and detection process strictly. The quality control before clinical sample analysis can make the test result more accurate and reliable.
【作者单位】：广东省工伤康复医院检验科;
【分类号】：R446.11
【正文快照】： 标本溶血的原因很多,但主要原因是指医护人员由于采血操作不当或临床检验操作不规范造成红细胞结构破坏,导致某些物质进入血清中,使标本呈现红色[1],血小板、白细胞、红细胞等血细胞破坏所释放的某些细胞内成分对临床生化指标测定会形成干扰,是临床生化检验实验中最常见的一种

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