梅毒特异性抗体分离检测装置的性能验证和初步应用

发布时间：2018-02-01 15:06

本文关键词： 亲和层析梅毒螺旋体梅毒特异性IgG抗体梅毒特异性IgM抗体特异性抗体分型检测　出处：《上海交通大学学报(医学版)》2017年05期 　论文类型：期刊论文

【摘要】：目的·设计获得梅毒特异性抗体分离检测的免疫亲和层析装置,对其检测性能进行验证,并初步应用于临床。方法·通过亲和层析装置中包被梅毒螺旋体抗原的亲和填料,特异性吸附样品中可能存在的梅毒螺旋体特异性抗体(包括IgG和IgM型),经洗涤、洗脱、脱盐处理后,由羊抗人IgG、IgM金标层析试纸条检测可能存在的梅毒特异性IgG、IgM型抗体。对20例梅毒总抗体检测阴性标本、230例梅毒抗体阳性的临床标本进行检测,并对其中的40例标本进行免疫印迹检测法对比测定。结果·优化获得亲和层析装置标准化操作程序。该装置能有效实现梅毒特异性IgG、IgM型抗体的分离检测。20例梅毒总抗体检测阴性标本经该装置检测的结果均为阴性。230例梅毒抗体阳性的临床标本中,2例标本检测结果为TP-IgG、IgM型抗体均阴性,210例标本检测结果为TP-IgG型抗体阳性、TP-IgM型抗体阴性,10例标本检测结果为TP-IgG型抗体阴性、TP-IgM型抗体阳性,8例标本检测结果为TP-IgG、IgM型抗体均阳性。40例标本检测结果与免疫印迹检测法结果进行比对,其中2例标本亲和层析装置检测结果为TP-IgG、IgM型抗体均阴性,免疫印迹检测法结果均为TP-IgG型抗体阳性、TP-IgM型抗体阴性,但2种方法检测结果的差异无统计学意义(P0.05)。结论·设计获得的该亲和装置应用于临床样本中梅毒特异性IgM、IgG抗体的分离检测切实可行,对后续临床运用有重要价值。
[Abstract]:Objective: to design an immunoaffinity chromatography device for the isolation and detection of syphilis specific antibodies. Methods: the affinity filler coated with Treponema pallidum antigen was used in the affinity chromatography device. Treponema pallidum specific antibodies (including IgG and IgM type) which may exist in the specific adsorption samples were washed, eluted, desalted and treated with sheep anti-human IgG. IgM gold standard chromatographic test strip was used to detect the possible syphilis specific IgGG IgM antibody. Twenty cases of syphilis total antibody negative samples were tested for syphilis antibody positive clinical specimens. The results showed that the standard operating procedure of affinity chromatography device was optimized. This device could effectively realize syphilis specific IgG. Isolation and detection of IgM antibody. 20 cases of syphilis total antibody negative samples were detected by this device. The results were all negative. 230 cases of clinical specimens with positive syphilis antibody. The results of TP-IgG + IgM antibody were negative in 2 cases and 210 cases were negative for TP-IgG antibody positive and TP-IgM type antibody. The results of detection of 10 specimens were TP-IgG negative and TP-IgM antibodies were positive in 8 cases, the results were TP-IgG. The results of IgM antibody were compared with those of Western blotting in all 40 samples. The results of affinity chromatography in 2 cases were all negative. The results of immunoblotting were all positive for TP-IgG type antibody and negative for TP-IgM antibody. But there was no significant difference between the two methods. Conclusion the designed affinity device was applied to syphilis specific IgM in clinical samples. The separation and detection of IgG antibody is feasible and valuable for clinical application.
【作者单位】：上海交通大学医学院附属瑞金医院检验科;
【基金】：上海市科学技术委员会项目(14411964500)~~
【分类号】：R446.6;R759.1
【正文快照】： 梅毒(syphilis)是由密螺旋体属(Treponema)中的梅毒螺旋体(Treponema pallidum,TP)引起的一种慢性、系统性性传播疾病(sexually transmitted disease,STD)。近年来,梅毒又引起临床密切关注,其快速增长的发病人数仅次于肺结核、肝炎,居全国乙类法定传染病发病数的第三位。预防

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