基于硅纳米球探针构建的超灵敏电致化学发光免疫传感器用于丙肝核心抗原检测与临床诊断

发布时间：2018-02-28 21:31

本文关键词： SiO@Ru纳米复合物 HCVcAg 夹心法免疫检测电致化学发光　出处：《南京医科大学学报(自然科学版)》2017年10期 　论文类型：期刊论文

【摘要】：目的:制备硅纳米球包裹的Ru(bpy)_3~(2+)(SiO_2@Ru)作为电致化学发光(electrogenerated chemiluminescence,ECL)探针的生物传感器,评价其在丙型肝炎核心抗原(HCVcAg)检测中的应用价值。方法:采用"油包水"的微乳剂方案,制备SiO_2@Ru纳米复合物,纳米粒子粒径一致,因此具有很好的重复性,应用戊二醛作为交联剂实现抗体与硅球的交联。SiO_2@Ru可作为ECL探针,通过夹心法检测HCVcAg。结果:HCVcAg检测的线性范围为1~1 000 fmol/L,最低检出限为3 fmol/L。与临床HCV RNA比较,有较好的相关性,线性相关系数R~2=0.937 9。结论 :构建HCVcAg的检测的硅纳米免疫传感器具有很高的灵敏度和很好的重复性,可用于临床诊断、疗效评价以及血液中心的血源筛查。
[Abstract]:Objective: to prepare silicon nanospheres coated with Ru(bpy)_3~(2 / SiO2@ Ru) as a biosensor for electrogenerated chemiluminescence (ECL) probes, and evaluate its application value in the detection of hepatitis C core antigen (HCV-cAg). Methods: a "oil-in-water" microemulsion scheme was used. The preparation of SiO_2@Ru nanocomposites has the same particle size, so it has good reproducibility. Using glutaraldehyde as crosslinking agent, the cross-linking of antibody with silica spheres can be used as ECL probe. Results the linear range of detection of HCVcAg was 1 000 fmol / L, and the lowest detection limit was 3 fmol / L. Compared with clinical HCV RNA, there was a good correlation between the detection of HCVcAg and clinical HCVcAg.The linear range of detection of HCVcAg was 1 000 fmol / L, and the lowest detection limit was 3 fmol / L. Conclusion: the silicon nano-immunosensor for HCVcAg detection has high sensitivity and good repeatability, and can be used in clinical diagnosis, evaluation of curative effect and blood screening in blood center.
【作者单位】：解放军第105医院检验科;南京市六合区医院检验科;南京市第二医院中心实验室;
【基金】：南京市科学技术厅社会发展项目(201201066) 南京市医学科技发展项目(YKK15144)
【分类号】：R446.6;R512.63

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