家蚕的过敏原组分鉴定

发布时间：2018-03-06 07:18

本文选题：家蚕　切入点：过敏原　出处：《生物技术通讯》2017年02期 　论文类型：期刊论文

【摘要】：目的:分析引起蚕过敏的组分。方法:利用磷酸缓冲液提取蚕茧和蚕蛹的总蛋白,用SDS-PAGE分析蚕蛋白组分,将蛋白胶转印到PVDF膜上,用蚕过敏患者的阳性血清进行IgE结合试验来鉴定蚕茧和蚕蛹的过敏原组分。结果:蚕茧蛋白可辨条带有8条,其相对分子质量分别约为18×10~3、27×10~3、30×10~3、38×10~3、42×10~3、44×10~3、51×10~3和58×10~3;蚕蛹蛋白主带有6条,其相对分子质量分别约为23×10~3、27×10~3、30×10~3、33×10~3、38×10~3和51×10~3;蚕茧和蚕蛹在相对分子质量约30×10~3和51×10~3附近有2条印迹主带。结论:提示家蚕蛋白中存在特定的过敏原。该研究为蚕过敏的诊断和治疗提供了一定的实验基础。
[Abstract]:Objective: to analyze the components of silkworm allergy. Methods: the total protein of silkworm cocoon and silkworm pupa was extracted by phosphoric acid buffer solution, the protein component was analyzed by SDS-PAGE, and the protein glue was transferred to PVDF film. The allergen components of cocoon and pupae were identified by IgE binding test with positive serum of silkworm allergy patients. Results: the protein of cocoon was identified with 8 bands, the relative molecular weight of which was about 18 脳 10 ~ (327) 脳 10 ~ (3) ~ (3) ~ 30 脳 10 ~ (38) 脳 10 ~ (338) 脳 10 ~ (338) 脳 10 ~ (344) 脳 10 ~ (351) 脳 10 ~ (3) and 58 脳 10 ~ (3) of silkworm pupae protein. Its relative molecular weight is about 23 脳 10 ~ (3) C ~ (3) ~ (27) 脳 10 ~ (10) ~ (3) ~ (30) 脳 10 ~ (3) C ~ (33) 脳 10 ~ (3) C ~ (3) and 51 脳 10 ~ (3) of silkworm pupae, and there are two imprinted main bands near the relative molecular weight of 30 脳 10 ~ (-3) and 51 脳 10 ~ (3) of silkworm pupae. Conclusion: there is a specific allergen in silkworm protein, and this study is a diagnostic method of silkworm allergy. Treatment provides a certain experimental basis.
【作者单位】：惠州学院生命科学学院;
【基金】：惠州市科技计划(2006P45) 惠州学院校立项目(C206·0209)
【分类号】：R446.6

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