抗核抗体两种检测方法在系统性红斑狼疮中的诊断性能比较

发布时间：2018-03-08 10:27

本文选题：抗核抗体　切入点：系统性红斑狼疮　出处：《实用医学杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的:采用酶联免疫吸附技术(ELISA)和线性免疫印迹技术(LIA)检测患者血清中的抗核抗体(ANA),评价和比较两种方法辅助诊断系统性红斑狼疮(SLE)的性能。方法:收集近两年同时使用两种方法检测血清ANA的病例共597例,记录检测结果及临床诊断,进行回顾性分析,分别统计两种方法针对SLE的诊断性能指标,包括:灵敏度、特异度、准确度、阳性预测值、阴性预测值,并进行统计学检验;分析ANA两种检测方法的检测结果的一致性。结果:ELISA法与LIA法检测ANA用于诊断SLE的灵敏度分别为79.3%、86.6%,特异度分别为81.7%、77.3%,准确度分别为81.4%、78.6%,阳性预测值分别为40.9%、37.8%,阴性预测值分别为96.1%、97.3%,两种方法的以上诊断性能指标均无统计学差异(P0.05);两种方法检测结果符合率为81.4%,判断一致性κ=0.55,具备较好的一致性;在SLE组中两种方法的一致性κ=0.403。结论:ELISA法和LIA法检测ANA在系统性红斑狼疮中的诊断性能无统计学差异,联合应用两种检测方法具有重要的应用价值。
[Abstract]:Objective: to evaluate and compare the performance of two methods for the diagnosis of systemic lupus erythematosus (SLE) by Elisa and LIA. At the same time, 597 cases of serum ANA were detected by two methods. To record the test results and clinical diagnosis, and make retrospective analysis, respectively, statistics two methods for SLE diagnostic performance indicators, including: sensitivity, specificity, accuracy, positive predictive value, negative predictive value, and statistical test; Results the sensitivity, specificity, accuracy, positive predictive value, negative predictive value and negative predictive value of ANA were 79.3 and 86.6, 81.7, 77.3, 81.4, 40.9, and 37.8, respectively, respectively. The sensitivity of the two methods was 79.36.6. the specificity was 77.3, the accuracy was 81.47.6, the positive predictive value was 37.8, and the negative predictive value was 37.83.Results the sensitivity of the two methods for the detection of ANA was 79.36.6. There was no statistical difference in the above diagnostic performance indexes between the two methods (P 0.05), the coincidence rate of the two methods was 81.4, the judgment consistency was 0.55, and the two methods had good consistency. Conclusion there is no statistical difference between the two methods in the diagnosis of systemic lupus erythematosus (SLE), and the combined application of the two methods is of great value in the diagnosis of systemic lupus erythematosus.
【作者单位】：广州医科大学附属第一医院检验科;
【分类号】：R446.6;R593.241

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