不同芽孢杆菌的太赫兹时域光谱特征研究

发布时间：2018-03-13 17:50

本文选题：太赫兹时域光谱　切入点：芽孢杆菌　出处：《第三军医大学学报》2017年13期 　论文类型：期刊论文

【摘要】：目的应用太赫兹时域光谱技术对常见致病芽孢杆菌进行检测。方法使用太赫兹时域光谱技术对枯草芽孢杆菌、蜡状芽孢杆菌、苏云金芽孢杆菌3种致病菌进行了室温、干燥环境下太赫兹时域光谱测试。得到了样品在0.22.0 THz波段的太赫兹吸收曲线。结果随着频率增加不同含量的芽孢杆菌冻干粉的太赫兹波段的吸收系数是增加的,并且有不同的吸收趋势;此外不同种属的芽孢杆菌的冻干粉和菌落也具有明显不同的吸收系数,这意味着3种样品的分子及官能团的构成和存在状态是不同的,更有力地揭示3种样品的确具有不同的生物成分组成和结构。结论太赫兹时域光谱技术作为一种快速高效、无需标记且无破坏性的探测方法,为芽孢杆菌的检测和鉴别提供了一种行之有效的方法。
[Abstract]:Objective to detect common pathogenic Bacillus by terahertz time domain spectroscopy (THz). Methods three pathogenic bacteria, Bacillus subtilis, Bacillus cereus and Bacillus thuringiensis, were detected at room temperature by terahertz time domain spectroscopy. The terahertz absorption curves of samples in 0.22.0 THz band were obtained by terahertz time-domain spectroscopy measurement in dry environment. Results the absorption coefficients of the terahertz band of Bacillus lyophilized powder with different contents of Bacillus lyophilized powder increased with the increase of frequency. In addition, the freeze-dried powder and colony of different species of Bacillus also have different absorption coefficients, which means that the composition and existence of the molecular and functional groups of the three samples are different. The results show that the three samples do have different biological composition and structure. Conclusion terahertz time-domain spectroscopy is a fast, efficient, non-tagging and non-destructive detection method. It provides an effective method for the detection and identification of Bacillus.
【作者单位】：第三军医大学西南医院检验科;中国人民解放军第三二四医院检验科;
【基金】：国家重点基础研究发展计划项目(2015CB755400,2015CB755402) 国家自然科学基金重点项目(81430054) 军队技术产品研究项目重点项目(BWS13C013)~~
【分类号】：R446.5

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