MiR205在脓毒症小鼠心脑肾中对HMGB1表达的影响

发布时间：2018-03-16 07:15

本文选题：miR-　切入点：脓毒症　出处：《武汉大学学报(医学版)》2017年03期 　论文类型：期刊论文

【摘要】：目的:检测在胆碱能抗炎通路(CAP)中miR-205表达变化和对脓毒症小鼠脑、心、肾组织中高迁移率族蛋白B1(HMGB1)的调节作用。方法:15只BALB/C小鼠被随机分成3组:脓毒症组和CAP组各6只,对照(control)组3只,分别腹腔注射脂多糖(LPS)15mg/kg+生理盐水,LPS 15mg/kg+GTS-21 4mg/kg,等量生理盐水。24h后处死小鼠取材心、肾、脑组织。定量逆转录聚合酶链反应(qRT-PCR)和酶联免疫吸附试验(ELISA)检测脓毒症小鼠和胆碱能抗炎通路小鼠的脑、心、肾组织中miR-205与HMGB1的表达。结果:脓毒症小鼠脑、心、肾组织的miR-205表达与对照组相比无明显变化(P_2、P_4、P_60.05),CAP组均较其他两组明显升高(P_1、P_3、P_50.001)。脑、肾组织中HMGB1的表达CAP组较脓毒症组有明显降低(P_1、P_30.001)。心肌组织中HMGB1的表达在脓毒症组和CAP组差异无显著性(P50.05)。结论:激活胆碱能抗炎通路时,脑、心、肾组织中表达升高的miR-205降低脑、肾组织中HMGB1的表达。
[Abstract]:Objective: to investigate the changes of miR-205 expression in cholinergic anti-inflammatory pathway (CAP) and its regulatory effects on the brain, heart and kidney tissues of septic mice. Methods: fifteen BALB/C mice were randomly divided into three groups: sepsis group and CAP group (n = 6 each). The control group (n = 3) received intraperitoneal injection of lipopolysaccharide (LPS) 15 mg / kg normal saline (LPS 15 mg / kg GTS-21 4 mg / kg). Brain tissue. Quantitative reverse transcriptase polymerase chain reaction qRT-PCR and enzyme linked immunosorbent assay (Elisa) were used to detect the expression of miR-205 and HMGB1 in brain, heart and kidney of septic mice and cholinergic anti-inflammatory pathway mice. The expression of miR-205 in renal tissue was not significantly changed compared with that in control group. The expression of miR-205 in the group of P2P _ 4 and P _ S _ 4 / P _ P _ 0. 05 / P was significantly higher than that in the other two groups, and the expression of miR-205 in the renal tissue was significantly higher than that in the other two groups. The expression of HMGB1 in renal tissue in CAP group was significantly lower than that in septic group. The expression of HMGB1 in myocardial tissue was not significantly different between septic group and CAP group. Conclusion: when activating cholinergic anti-inflammatory pathway, the expression of miR-205 in brain, heart and kidney decreased. Expression of HMGB1 in renal tissue.
【作者单位】：武汉科技大学城市学院医学部;武汉大学中南医院重症医学科;
【基金】：国家自然科学基金资助项目(编号:30972852)
【分类号】：R459.7

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