抗核小体抗体对儿童系统性红斑狼疮诊断的应用价值

发布时间：2018-03-20 09:32

本文选题：系统性红斑狼疮　切入点：抗核小体抗体　出处：《临床检验杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的评价抗核小体抗体(Anu A)对儿童系统性红斑狼疮(JSLE)诊断的应用价值。方法选取54例JSLE患者,28例非JSLE患者及26例健康人作为研究对象,采用酶联免疫法检测抗核抗体,免疫印迹法检测抗核小体抗体、抗ds DNA抗体、抗组蛋白抗体及抗Sm抗体;同时收集临床资料及相关实验室检查结果,并进行相关性分析。结果 Anu A对JSLE诊断的敏感性为77.78%,特异性为96.30%;Anu A并联抗核抗体、抗ds DNA抗体及抗Sm抗体检测诊断JSLE的敏感性高于单项检测;Anu A与发热、口腔/鼻咽部溃疡、肺部损害、淋巴细胞绝对值、尿蛋白和补体C3正相关。结论 Anu A可作为诊断JSLE有价值的指导性血清标志物,其联合抗核抗体、抗ds DNA抗体及抗Sm抗体检测更有利于JSLE疾病的诊断。
[Abstract]:Objective to evaluate the value of anti-nucleosome antibody (Anu) in the diagnosis of children with systemic lupus erythematosus (JSLE). Methods A total of 54 patients with JSLE (n = 28) and 26 healthy controls (n = 26) were selected for the detection of antinuclear antibodies (ANA) by enzyme-linked immunosorbent assay (Elisa). Western blot was used to detect anti-nucleosome antibody, anti-DS DNA antibody, anti-histone antibody and anti-Sm antibody. Results the sensitivity of Anu A to the diagnosis of JSLE was 77.78 and the specificity was 96.30. The sensitivity of Anu A parallel anti-nuclear antibody, anti-DS DNA antibody and anti-Sm antibody in the diagnosis of JSLE was higher than that of single detection of Anu A and fever, oral / nasopharyngeal ulcer. Pulmonary damage, absolute value of lymphocytes, urinary protein and complement C3 were positively correlated. Conclusion Anu A can be used as a valuable guiding serum marker for the diagnosis of JSLE, and combined with antinuclear antibody. The detection of anti DS DNA antibody and anti Sm antibody is more helpful for the diagnosis of JSLE disease.
【作者单位】：中山大学附属中山市人民医院检验医学中心;
【分类号】：R725.9;R446.6

【参考文献】