临床分离大肠埃希菌质粒介导喹诺酮耐药基因和β内酰胺酶基因检测

发布时间：2018-03-22 20:01

本文选题：大肠埃希菌　切入点：喹诺酮类耐药　出处：《中国感染与化疗杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的探讨大肠埃希菌临床分离株质粒介导喹诺酮耐药(PMQR)基因的流行情况,及其与β内酰胺酶基因相关性。方法收集2013年7-12月福建医科大学附属协和医院临床分离的对左氧氟沙星和/或环丙沙星耐药的大肠埃希菌200株,采用PCR检测qnrA、qnrB、qnrC、qnrD、qnrS、aac(6')-Ib-cr、qepA和oqxAB基因,对PMQR基因阳性菌株扩增常见β内酰胺酶基因;采用琼脂稀释法对PMQR基因阳性菌株进行药敏试验,以及PCR法对菌株进行系统发育分型;利用肠杆菌基因重复一致序列分析(ERIC-PCR)进行菌株同源性分析。结果 PCR扩增结果显示实验菌株PMQR阳性率为29.0%(58/200)。其中qnr阳性率为5.5%(11/200),aac(6')-Ib-cr阳性率为20.5%(41/200),oqx AB阳性率为8.0%(16/200),qepA阳性率为0.5%(1/200)。58株PMQR基因阳性菌株中CTX-M-1组32株(55.2%)、CTX-M-9组17株(29.3%)和TEM型1株(1.7%),未检出SHV型β内酰胺酶基因。PMQR阳性菌呈现多重耐药现象;系统发育分型结果显示A型有21株(36.2%),D型17株(29.3%),B2型11株(19.0%),B1型9株(15.5%)。ERIC-PCR显示PMQR大肠埃希菌可分为50个不同的型别,其中1株未能分型。结论该院大肠埃希菌中PMQR基因以aac(6')-Ib-cr、qnr和oqxAB为主,且与β内酰胺酶耐药基因高度相关;此外PMQR菌株以非克隆播散方式在该院中流行。
[Abstract]:Objective to investigate the prevalence of quinolone-resistant PMQR gene in clinical isolates of Escherichia coli. Methods from July to December 2013, 200 strains of Escherichia coli resistant to levofloxacin and / or ciprofloxacin were collected and detected by PCR. The common 尾 lactamase genes were amplified from PMQR gene positive strains, the susceptibility test of PMQR gene positive strains was carried out by Agar dilution method, and the phylogenetic typing of PMQR gene positive strains was carried out by PCR method. The results of PCR amplification showed that the positive rate of PMQR of the experimental strain was 29.0 / 58 / 2000.The positive rate of qnr was 5.555% and the positive rate of qnr was 20.50.41 / 200oqx AB was 8.0 / 162000.58%. Among the positive strains of PMQR gene, 32 strains of CTX-M-1 group and 17 strains of CTX-M-9 group were found to be multidrug resistant to SHV 尾 lactamase gene. The results of phylogenetic typing showed that there were 21 strains of type A and 17 strains of type D (36.2D), and 11 strains of type B _ 2 (19.0B _ 2) showed that 9 strains of type B _ 1 were divided into 50 different types, one of which could not be genotyped. Conclusion the PMQR gene of Escherichia coli in this hospital is mainly aacaa6ke-Ib-crrqnr and oqxAB. Moreover, PMQR strains were highly associated with 尾 -lactamase resistance genes, and PMQR strains were prevalent in the hospital.
【作者单位】：福建医科大学附属协和医院检验科;福建医科大学医学与技术工程学院;
【基金】：国家自然科学基金(81201328) 福建省高校杰青项目(JA13134) 福建省卫计委中青年骨干人才培养项目(2015-ZQN-ZD-15)
【分类号】：R446.5

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