大鼠肝脏缺血再灌注模型超高b值MR扩散成像与水通道蛋白-4表达的相关性研究

发布时间：2018-03-23 12:35

本文选题：肝　切入点：再灌注损伤　出处：《中国临床医学影像杂志》2017年10期

【摘要】：目的 :研究水通道蛋白-4(Aquaporin-4,AQP-4)在大鼠肝脏缺血再灌注模型中的表达规律,探讨此模型超高b值磁共振扩散加权成像(Diffusion weighted imaging,DWI)与AQP-4表达的相关性。方法:选取体质量为250~300 g的SD大鼠42只,分为7组,每组6只。实验组行肝右叶门静脉和肝动脉结扎3 h后再灌注,对照组行假手术。两组分别在再灌注后0 h、6 h、12 h、1 d、3 d、7 d和14 d行3.0T MRI扫描,包括平扫T_1WI和T_2WI、常规DWI(0 s/mm~2,1 000 s/mm~2)和15个b值DWI(0~4 500 s/mm~2)扫描,分别根据单b值DWI和15个b值DWI测量记录标准ADC值(Standard apparent diffusion coefficient,ADC_s)以及超高ADC值(Ultra-high apparent diffusion coefficient,ADC_u)。观察各时间点各参数的动态变化规律。扫描后给予动物安乐死,留取肝脏标本用于HE染色,光镜下观察肝脏病理形态学变化、蛋白质印迹法(Western blot)观察AQP-4表达变化。结果:实验组在术后12 h开始至3 d ADC_s值均高于对照组,术后7 d开始恢复正常。实验组ADC_u在术后6 h开始比对照组明显增高,术后14 d恢复正常。实验组AQP-4表达量在术后6 h、12 h、1 d、3 d逐渐降低但均高于对照组,术后7 d仍减低并开始接近正常。ADC_s与AQP-4表达量不具有相关性(P=0.416),而ADC_u与AQP-4表达量具有相关性(P=0.034),相关系数为0.721。结论:超高b值DWI可以反映AQP的转运情况。
[Abstract]:Objective: to study the expression of aquaporin-4 aquaporin-4AQP-4 in rat liver ischemia-reperfusion model. To investigate the correlation between DWI and AQP-4 expression in hyperbaric magnetic resonance diffusion weighted imaging (DWI). Methods: 42 SD rats with volume weight of 250,300g were selected and divided into 7 groups. There were 6 rats in each group. The right hepatic portal vein and hepatic artery were ligated for 3 h after reperfusion in the experimental group and the sham operation was performed in the control group. The rats in the two groups were scanned with 3.0T MRI at 0 h, 12 h, 12 h, 1 day and 3 d after reperfusion for 7 d and 14 d, respectively. Including plain scan T_1WI and T2WI, conventional DWI(0 s / mmm 21000 s / mm2) and 15 b DWI(0~4 500s / mmm2) scans, Standard apparent diffusion efficient cells were measured according to single b-value DWI and 15 b-valued DWI. The dynamic changes of parameters at each time point were observed. After scanning, animals were euthanized and liver specimens were taken for HE staining. The expression of AQP-4 was observed by Western blot. Results: the ADC_s value of the experimental group was higher than that of the control group from 12 hours to 3 days after operation. ADC_u in the experimental group was significantly higher than that in the control group at 6 hours after operation and returned to normal at 14 days after operation. The expression of AQP-4 in the experimental group gradually decreased at 12 hours after operation, but was higher than that in the control group at 1 and 3 days after operation. On the 7th day after operation, there was no correlation between the expression of DWI and AQP-4, but there was a correlation between the expression of ADC_u and AQP-4, the correlation coefficient was 0.721. Conclusion: the ultrahigh value of DWI can reflect the transport of AQP.
【作者单位】：浙江大学医学院附属第一医院放射科;浙江大学数学科学学院;
【基金】：国家自然科学基金面上项目(30670603) 浙江省卫计委培育项目(2014PYA009)
【分类号】：R445.2;R575

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