低声压级次声对神经病理性疼痛大鼠脊髓趋化因子介导炎症反应的影响

发布时间：2018-03-27 22:36

本文选题：神经病理性疼痛　切入点：次声　出处：《临床麻醉学杂志》2017年09期

【摘要】：目的探讨低声压级次声对神经病理性疼痛大鼠脊髓趋化因子介导炎症反应的影响。方法 72只健康雄性SD大鼠采用随机数字表分为三组,假手术组(Sham组)、神经病理性疼痛组(NP组)和低声压级次声干预组(LNP组),每组24只。制备大鼠坐骨神经慢性压迫性损伤模型。Sham组大鼠仅暴露坐骨神经而不结扎,其余操作均与NP组和LNP组相同。LNP组大鼠于造模后第4天开始接受低声压级次声治疗,每天4h,连续进行21d。所有大鼠均于术前1d(T_0)和术后3d(T_1)检测机械缩足阈值(MWT),分别于术后10d(治疗7d,T_2)、17d(治疗14d,T_3)和24d(治疗21d,T_4),每组各随机取8只大鼠检测MWT,实时荧光定量PCR技术检测脊髓组织中单核细胞趋化蛋白-1(MCP-1)、趋化因子受体2(CCR2)、TNF-α、IL-1β和IL-6基因表达,Western blot法检测脊髓组织中MCP-1和CCR2蛋白含量。结果 T_1~T_4时NP组和LNP组大鼠MWT明显低于T_0时和Sham组,且LNP组大鼠MWT明显高于NP组(P0.05)。T_2~T_4时NP组和LNP组大鼠脊髓组织中MCP-1、CCR2、TNF-α、IL-1β和IL-6mRNA相对表达量明显高于Sham组(P0.05);T_3、T_4时LNP组大鼠脊髓组织中MCP-1、CCR2、TNF-α、IL-1β和IL-6mRNA相对表达量明显低于NP组(P0.05)。T_2~T_4时NP组和LNP组脊髓组织中MCP-1和CCR2蛋白含量明显高于Sham组,T_3、T_4时LNP组脊髓组织中MCP-1和CCR2蛋白含量均明显低于NP组(P0.05)。结论低声压级次声可有效缓解神经病理性疼痛大鼠疼痛症状,机制可能与抑制趋化因子介导的级联炎症反应有关。
[Abstract]:Objective to investigate the effect of infrasound with low sound pressure on inflammatory response mediated by chemokines in spinal cord of rats with neuropathic pain. Methods 72 healthy male SD rats were randomly divided into three groups. Sham group, neuropathic pain group (NP group) and low sound pressure infrasound intervention group (LNP group, 24 rats in each group). The model of chronic compression injury of sciatic nerve in rats. Sham group only exposed sciatic nerve without ligation. The other operations were the same as those in NP group and LNP group. The rats in LNP group received low sound pressure infrasound therapy on the 4th day after modeling. At 4 hours per day, 21 consecutive days. All the rats were measured at 1 day before operation and 3 days after operation. The threshold of mechanical foot contraction was measured at 10 days after operation (7 days after treatment) and 24 days after treatment. Each group was randomly selected to detect MWTs and real-time fluorescence quantitative PCR (real-time fluorescence quantitative PCR), respectively, at 17 days after operation (treatment of 14 days after treatment) and 24 days (treatment of 21 days after treatment with T 4), and 8 rats from each group were randomly selected for detection of MWTs and real-time fluorescence quantitative PCR technique, respectively, at 10 days after operation (treatment 7 days after treatment) and 24 days after treatment (treatment of 21 days after treatment with T4). The expression of monocyte chemoattractant protein (MCP-1), chemokine receptor 2CCR2TNF- 伪 TNF- 伪 IL-1 尾 and IL-6 in spinal cord tissue were detected by Western blot. Results the MWT of NP group and LNP group were significantly lower than those of T0 and Sham groups at T_1~T_4. The relative expressions of MWT and IL-6mRNA in the spinal cord of NP group and LNP group were significantly higher than those in NP group (P 0.05) and LNP group (P < 0.01). The relative expressions of IL-6mRNA and MCP-1CCR2TNF- 伪 IL-1 尾 and IL-6mRNA in LNP group were significantly lower than those in NP group (P 0.05) and T _ 2T _ 4 group at P 0.05n. T2T _ 4 time after treatment, and the relative expression of MCP-1CCR2TNF- 伪 IL-1 尾 and IL-6mRNA in LNP group were significantly lower than those in NP group and T _ 2T _ 4 group. The content of MCP-1 and CCR2 protein in spinal cord in LNP group was significantly higher than that in Sham group TST3 / T4. The contents of MCP-1 and CCR2 protein in spinal cord in LNP group were significantly lower than those in NP group (P 0.05). Conclusion low sound pressure infrasound can effectively relieve the pain symptoms of neuropathic pain rats. The mechanism may be related to the inhibition of chemokine-mediated cascade inflammation.
【作者单位】：河南省南阳市中心医院;郑州大学附属郑州中心医院;
【基金】：河南省2014年科技发展计划(142102310459)
【分类号】：R402

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