D-二聚体胶体金免疫层析定量检测方法的初步建立

发布时间：2018-04-01 12:30

本文选题：D-二聚体　切入点：胶体金免疫层析　出处：《河南医学研究》2017年02期

【摘要】：目的建立一种检测D-二聚体的胶体金免疫层析定量检测方法,为临床检测D-二聚体提供一种简便、快速、准确的检测方法。方法根据免疫学中双抗体夹心和免疫层析的原理,研制定量检测样本中D-二聚体的胶体金试纸条,在金标定量仪上建立标准曲线,从灵敏度、特异性、线性、精密性、稳定性和方法学对比等方面对该方法进行评价。结果本试纸条的线性范围为0.156 25~10μg/ml,回归方程Y=92.907 X+307.88,R2=0.982 68;批内变异系数(CV)为3.78%和2.81%,批间变异系数(CV)为4.95%和4.07%;与纤维蛋白原无交叉反应性;在37℃条件下保存1周;与IL公司ACL-TOP700检测系统免疫比浊法的相关性良好,回归方程Y=0.959 64 X+0.026 45,相关系数R2=0.985 93。结论本研究初步建立了检测D-二聚体的胶体金免疫层析定量检测方法,为临床检测D-二聚体提供了一种新的技术和方法。
[Abstract]:Objective to establish a colloidal gold immunochromatographic quantitative detection method for detecting D- two dimers, for the clinical detection of D- two dimer provides a simple, rapid and accurate detection method. The method is based on the principle of double antibody sandwich in immunology and immune chromatography, preparation of colloidal gold detection samples two D- dimer test strip in the gold standard, quantitative instrument to establish the standard curve, the sensitivity, specificity, linearity, precision, stability and other aspects of the evaluation method comparison of the proposed method. Results the linear range of the test strip was 0.156 25~10 g/ml, the regression equation is Y= 92.907 X+307.88, R2=0.982 68; the intraassay coefficient of variation (CV) 3.78% and 2.81%, coefficient of variation between groups (CV) were 4.95% and 4.07%; no cross reactivity with fibrin; stored for 1 weeks under the condition of 37 DEG C; and IL ACL-TOP700 immune detection system than good correlation method, the regression equation of Y=0.959 64 X+0 .026 45, correlation coefficient R2=0.985 93. conclusion this study preliminarily established a colloidal gold immunochromatography quantitative detection method for detecting D- two polymers, which provided a new technology and method for clinical detection of D- two polymers.

【作者单位】：郑州大学第一附属医院;郑州职业技术学院;河南省生物工程技术研究中心;
【分类号】：R446.6

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