SPIO-shRNA分子探针用于荷瘤裸鼠MR检查的最佳扫描浓度探讨

发布时间：2018-04-14 17:34

  本文选题：超顺磁性氧化铁 + 分子探针　； 参考：《东南大学学报(医学版)》2017年03期

【摘要】：目的:探讨SPIO-shRNA分子探针在活体荷瘤裸鼠肿瘤区域组织信号的变化情况,阐明分子探针用于荷瘤裸鼠的最佳扫描浓度。方法:将30只BALB/c荷瘤裸鼠随机分为5组,每组6只,在既定扫描时间(探针注射前及注射后27 h)分别以含铁量为6、12、18、24、30 mg·kg~(-1)尾静脉注射并同步行MR检查,测量肿瘤组织及对侧对应区域肌肉组织在各浓度组的信号强度变化。每次检查完成后立即处死裸鼠,取肿瘤组织及肌肉组织行HE及普鲁士蓝染色,并在光镜下观察。结果:6、12、18 mg·kg~(-1)组裸鼠探针注射后均存活,24、30 mg·kg~(-1)组探针注射后及扫描过程中出现部分裸鼠死亡;各浓度组间肿瘤组织体积差异无统计学意义(P0.05);MR各扫描序列以T_2WI及T_2*序列的信号改变最明显;各浓度组比较,肿瘤组织信号强度在18、24、30 mg·kg~(-1)组较其余组差异明显(P0.05),而18、24、30 mg·kg~(-1)组间差异无统计学意义(P0.05);HE染色显示肿瘤组织结构紊乱,细胞核异型性较多;普鲁士蓝染色表明不同浓度组肿瘤组织中均存在蓝染的铁颗粒,其中18、24、30 mg·kg~(-1)组蓝染颗粒分布密集。结论:SPIO-shRNA分子探针能成功靶向于肿瘤组织,且最佳扫描浓度(含铁量)为18 mg·kg~(-1)。
[Abstract]:Aim: to investigate the changes of SPIO-shRNA molecular probe signal in the tumor tissue of nude mice bearing tumor in vivo and to elucidate the optimal scanning concentration of the molecular probe in nude mice bearing tumor.Methods: thirty nude mice with BALB/c tumor were randomly divided into 5 groups, 6 in each group. At the fixed scanning time (before injection and 27 hours after injection), the mice were injected intravenously with iron content of 612 ~ (18) and 2430 mg / kg / kg ~ (-1) respectively.The signal intensity of tumor tissue and contralateral muscle tissue in each concentration group was measured.The nude mice were killed immediately after each examination. The tumor tissues and muscle tissues were stained with HE and Prussian blue and observed under light microscope.Results all the nude mice in the control group survived 24 ~ 30 mg 路kg ~ (-1) after injection, and some of the nude mice died during the scanning and after the injection of the probe (30 mg 路kg ~ (-1)).There was no significant difference in the volume of tumor tissue among different concentration groups. The signal changes of T_2WI and T _ s _ 2 * sequences were the most obvious in Mr sequences of P0.05 Mr.The signal intensity of tumor tissue was significantly different from that of the other groups (P 0.05), but there was no significant difference between the two groups (P 0.05). The results of HE staining showed that the tumor tissue structure was disordered and the nuclear heterogeneity was higher.Prussian blue staining showed the presence of blue-stained iron particles in tumor tissues of different concentrations, and the distribution of blue-stained iron particles was dense in the 1824 ~ (24) mg / kg ~ (-1) group.Conclusion the molecular probe of SPIO-shRNA can be successfully targeted at tumor tissue, and the best scanning concentration (iron content) is 18 mg / kg ~ (-1) 路kg ~ (-1) 路L ~ (-1).
【作者单位】： 重庆医科大学附属第一医院放射科;
【基金】：国家自然科学基金资助项目(81171366) 国家临床重点专科建设项目(国卫办医函2013-544)
【分类号】：R445.2
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本文编号：1750366